2-(1-丟民伙巨玄平扑)巨正用□伙及
民乓奶瓦□朮’甩丞旦正□メ陃稱辿毛迕中月癆縉蟲唱橘魂董

In Vitro Chromosomal Aberration Test
of 2-(1-Methylethoxy)ethanol in Cultured Chinese Hamster Cells

邰沶

2-(1-丟民伙巨玄平扑)巨正用□伙及メ陃稱辿卞菴廿允稱辿唬鰻喀讀排黍卞勾中化﹞民乓奶瓦□朮’甩丞旦正□メ陃稱辿(CHL/IU)毛迕中化癆縉蟲唱橘魂董毛撢雒仄凶‘

稱辿餵縛俅孺魂董瑛絆毛手午卞﹞羶凜棉質咥芊匹10 mM鍬癲及タ蘸毛殖戈263﹞525﹞1050 米g/mL及3迕恟毛澀爛仄凶‘S9 mix繡箕票云方太�繡箕票匹6凜棉質咥(18凜棉及莢汊凜棉)詨﹞伓呿毛綜擠仄﹞腹黃允月仇午卞方曰癆縉蟲唱橘芘�嶺毛腹④仄凶‘

公及瑛絆﹞中內木及質咥歇卞云中化手﹞癆縉蟲及厭瞻唱橘丐月中反ム醒嶺稱辿及芘�綜迕反カ戶日木卅井勻凶‘蝨勻化﹞263﹞525﹞1050 米g/mL及3迕恟毛迕中凶S9 mix�繡箕票匹及洘糧質咥芊24凜棉質咥匹癆縉蟲唱橘魂董毛撢雒仄凶‘⑺魂董芊卞云中化手癆縉蟲及厭瞻唱橘丐月中反ム醒嶺稱辿及芘�綜迕反カ戶日木卅井勻凶‘

動曉及瑛絆方曰﹞呿魂董橢瘀票匹反2-(1-丟民伙巨玄平扑)巨正用□伙反﹞癆縉蟲唱橘毛芘�仄卅中(悻嶺)午瑛狨仄凶‘

杅芊

1.﹛魂董稱辿堪

荇し挀メ陃稱辿毛迕中月癆縉蟲唱橘魂董卞僮仁銀迕今木化中月仇午井日﹞魂董稱辿堪午仄化民乓奶瓦□朮’甩丞旦正□及ポ苀侳及瞬問舂稱辿堪(CHL/IU)毛薊鎗仄凶‘噯狟59コ11畸15ざ卞嶄峏啟戲魂董赭(蜇:嶄峏啣泔圴罹圴啟戲蒂菜赭)井日坌芨毛熬仃﹞斥丟民伙旦伙石平扑玉(DMSO:MERCK KGaA)毛10 vol%驕笛仄凶詨﹞敕蟲轎豳醱卞忡繡仄凶‘魂董卞端仄化反鑠瑛稱辿毛芼荸仄3℅5ざ仍午卞煤醫仄凶手及毛銀迕仄凶‘卅云﹞稱辿餵縛俅孺魂董匹反煤醫醒28丐月中反40(洘糧質咥芊)及稱辿毛﹞癆縉蟲唱橘魂董匹反30丐月中反12(洘糧質咥芊)及稱辿毛迕中凶‘

2.﹛メ陃敕及譬擠

Eagle-MEM敕蟲メ譁(偽氾弁用弘仿旦(堪))卞﹞丟件皮仿件白奴伙正□(0.45 米m:Featuring Corning and Costar Products)毛迕中化笛做葋聊輪嗜仄凶�⑸祭(56 ∵﹞30坌)碧心酴蛭瑜懦(GIBCO Life Technologies, Inc)毛瘉蔽タ蘸匹10 vol%卞卅月方丹笛尹凶詨﹞魂董卞銀迕仄凶‘譬擠詨及メ陃敕反挕剪赭(4 ∵)卞忡繡仄凶‘

3.﹛メ陃橢瘀

CO₂奶件平亙矛□正□(趕迓齬窗丟犯奴市扑旦氾丞(堪))毛迕中﹞CO₂タ蘸5 %﹞37 ∵及橢瘀匹稱辿毛メ陃仄凶‘

4.﹛S9 mix

擠瞻詨6仰畸動〇及平永戊□穴件(堪)擠S9 mix毛魂董卞銀迕仄凶‘S9 mix醱及S9反芘⑼管午仄化白尼用田伙申正□伙云方太5,6-矛件札白仿示件毛髑芨仄凶Sprague-Dawley煙芮仿永玄及棒癒井日譬擠仄凶‘引凶﹞S9 mix及賺嶽反噢痊日及杅芊¹⁾卞蝨勻凶‘S9 mix及賺嶽毛動票卞憎允‘

嶽坌	S9 mix 1 mL醱及恟
S9	0.3 mL
MgCl₂	5 米mol
KCl	33 米mol
G-6-P	5 米mol
NADP	4 米mol
HEPES森擋敕(pH 7.2)	4 米mol
橈帢踹	酸恟

5.﹛⻗董坁撙

2-(1-丟民伙巨玄平扑)巨正用□伙(伕永玄⺀寞:30321)反賬蘸99.5 %動曉(尕賬坁午仄化踹坌0.1 %動票毛殖戈)及⑨抸敕蟲匹丐月‘呿管反20 ∵及踹云方太橘硃匹及失本玄件卞敦蟈卞迍荸仄﹞踹迍敕醱匹兜爛匹丐月‘趕唳祭喀(堪)(鱗詁)井日羼間今木凶⻗董坁撙毛銀迕仄凶‘⻗董坁撙反﹞銀迕凜引匹撮硃匹忡棟仄凶‘魂董蔽弇詨﹞⻗董坁撙羼間葭卞云中化酸芴⻗董坁撙毛坌橾仄凶瑛絆﹞兜爛嶺卞杽鎖反卅井勻凶‘

6.﹛⻗董坁撙敕及譬擠

魂董及籥蘸﹞⻗董坁撙毛ざ呿泔傾杅鏜槨迕踹((堪)釐騵擠泔鼎樺)毛迕中化迍荸仄化譬擠葆敕午仄凶‘譬擠葆敕毛銀迕迍モ毛迕中化賜慼赭爛タ蘸卞渦澄仄凶詨﹞簧支井卞質咥毛墊勻凶‘

7.﹛稱辿餵縛俅孺魂董(芺ɛ魂董)

12它巨伙及稱辿メ陃迕穴伙民皿伊□玄卞稱辿毛ド潘仄﹞メ陃3ざ詨卞⻗董坁撙敕毛質咥仄凶‘羶凜棉質咥芊匹反S9 mix�繡箕票(-S9 mix)丐月中反繡箕票(+S9 mix)匹6凜棉質咥仄凶詨﹞蕙蟒卅メ陃敕卞跟晶仄化今日卞18凜棉メ陃毛糧仃凶‘洘糧質咥芊及樺寧﹞24凜棉洘糧仄化質咥毛撢雒仄凶‘

稱辿毛10 vol%醱嶺森擋石伙穴伉件敕(狟跪賬泔鼎僅(堪))匹裔爛仄凶詨﹞0.1 w/v%弁伉旦正伙’田奶左伊永玄(楮鱗祭喀(堪))踹迍敕匹10坌棉癆縉仄凶‘縉豳迍請敕(30 vol%巨正用□伙﹞1 vol%豫鄙踹迍敕)毛贗恟笛尹﹞5坌棉蠢蘸旵譆仄化縉豳毛迍請仄凶詨﹞580 nm匹及著跪蘸毛竅爛仄凶‘備迕恟歇卞勾中化迍モ覆寰歇匹及著跪蘸卞覆允月⺶﹞允卅歹切稱辿戲繡峔毛遙請仄凶‘

公及瑛絆﹞中內木及質咥芊卞云中化手抸割卅稱辿餵縛俅孺反棋說今木卅井勻凶(Fig. 1℅2)‘

卅云﹞⻗董坁撙佌洈蔽弇凜﹞pH及庍⑹﹞橾請羈及冂仵允屯五庍祭反﹞中內木及魂董迕恟卞云中化手棋說今木卅井勻凶‘

8.﹛魂董迕恟云方太魂董歇及澀爛

稱辿餵縛俅孺魂董瑛絆毛手午卞﹞癆縉蟲唱橘魂董匹反羶凜棉質咥芊-S9質咥卅日太卞+S9質咥午手1050 米g/mL(10 mM鍬癲)毛瘉嫖質咥タ蘸午仄﹞動票跤⺶2匹蛹元凶煌3迕恟卅日太卞迍モ覆寰歇毛澀爛仄凶‘

今日卞﹞癆縉蟲唱橘芘�卞楮仄化羶凜棉質咥芊匹悻嶺午�爛今木凶仇午井日﹞洘糧質咥芊24凜棉質咥卞云中化手1050 米g/mL(10 mM鍬癲)毛瘉嫖質咥タ蘸午仄﹞動票跤⺶2匹蛹元凶煌3迕恟毛澀爛仄凶‘

卅云﹞阺嶺覆寰午仄化﹞羶凜棉質咥芊及樺寧﹞-S9質咥匹穴奶玄穴奶扑件C(MMC:隅狟�夢鼎僅(堪))毛0.1 米g/mL﹞+S9質咥匹扑弁伕石旦白央立玉(CP:梅沓聒擠泔(堪))毛12.5 米g/mL及迕恟匹﹞洘糧質咥及樺寧MMC毛0.05 米g/mL及迕恟匹魂董仄凶‘

9.﹛癆縉蟲伓呿及綜擠

躂溘60 mm及皿伊□玄毛迕中﹞稱辿餵縛俅孺魂董午⑺迋卞⻗董坁撙羈及質咥毛墊勻凶‘メ陃蔽弇2凜棉蟆卞﹞瘉蔽タ蘸匹0.2 米g/mL午卅月方丹戊伙本立玉(GIBCO Life Technologies, Inc)毛驕笛仄凶‘玄伉皿扑件質咥匹稱辿毛ヱ峊今六﹞棄艘坌峊卞方曰稱辿毛莢璋仄凶‘75 mmol/L梅祭市伉它丞踹迍敕匹斕艦質咥毛墊勻凶詨﹞裔爛敕(丟正用□伙3芢:豫鄙1芢)匹稱辿毛裔爛仄凶‘塢竣揖鍋芊匹癆縉蟲伓呿毛綜擠仄凶詨﹞1.2 vol%幼丞扒癆縉敕匹12坌棉癆縉仄凶‘

10.﹛癆縉蟲及棋說

備皿伊□玄丐凶曰100蜊﹞允卅歹切迕恟癲凶曰200蜊及坌昴醱渝颶毛葡ɵ黃票匹棋說仄﹞癆縉蟲及溥謫讀庍祭午仄化幼乓永皿(gap)﹞癆縉坌蟲濠蠅(ctb)﹞癆縉蟲濠蠅(csb)﹞癆縉坌蟲跟晶(cte)﹞癆縉蟲跟晶(cse)云方太公及職(oth)及厭瞻唱橘卞坌挀仄凶‘⑺凜卞﹞ム醒嶺稱辿及請蜇峔毛筏狤仄凶‘癆縉蟲及坌橾反ざ呿棕雁庍唱葆喀莘’荇し⑹坁魂董坌粗莘卞方月坌挀芊²⁾卞蝨勻化撢雒仄凶‘

允屯化及伓呿毛戊□玉祭仄凶詨﹞癆縉蟲坌橾毛撢雒仄凶‘

11.﹛瑛絆及荸橾

幼乓永皿及心忡肣允月稱辿毛殖戶卅中樺寧(-gap)卞勾中化癆縉蟲厭瞻唱橘及請蜇奷蘸毛刓憎仄凶‘

備魂董歇及厭瞻唱橘毛肣允月稱辿丐月中反ム醒嶺稱辿及請蜇奷蘸毛﹞Fisher及躂濤割峔煌遙芊(肣啦踹賞0.05)毛迕中化腹爛仄凶‘引凶迕恟匙繡嶺卞勾中化反﹞Cochran Armitage及溯輊腹爛(肣啦踹賞0.05)毛迕中化腹爛仄凶‘迍モ覆寰歇午⺶勝仄⻗董坁撙質咥歇卞云中化肣啦犒互カ戶日木﹞井勾﹞瘋蜇嶺丐月中反迕恟卞匙繡嶺互カ戶日木凶樺寧卞阺嶺午�爛仄凶‘

瑛絆云方太嗶說

羶凜棉質咥芊匹及魂董瑛絆毛Table 1℅2卞憎仄凶‘2-(1-丟民伙巨玄平扑)巨正用□伙質咥歇及樺寧﹞羶凜棉質咥芊S9 mix�繡箕票云方太S9 mix繡箕票午手﹞中內木及迕恟卞云中化手癆縉蟲厭瞻唱橘卅日太卞ム醒嶺稱辿及芘�溯輊反棋說今木卅井勻凶‘引凶﹞10 mM鍬癲及 1050 米g/mL質咥歇卞云中化手葡難卅稱辿餵縛俅孺綜迕反棋說今木卅井勻凶‘羶凜棉質咥芊卞云中化悻嶺午�爛今木凶仇午井日﹞洘糧質咥芊24凜棉質咥卞方月癆縉蟲唱橘魂董毛撢雒仄﹞瑛絆毛Table 3卞憎仄凶‘洘糧質咥芊及中內木及迕恟卞云中化手癆縉蟲厭瞻唱橘卅日太卞ム醒嶺稱辿及芘�溯輊反棋說今木卅井勻凶‘引凶﹞10 mM鍬癲及1050 米g/mL質咥歇卞云中化手葡難卅稱辿餵縛俅孺綜迕反棋說今木卅井勻凶‘域杅﹞羶凜棉質咥芊S9 mix�繡箕票云方太洘糧質咥芊卞云仃月阺嶺覆寰坁撙MMC匹質咥仄凶稱辿﹞云方太羶凜棉質咥芊S9 mix繡箕票卞云仃月阺嶺覆寰坁撙CP匹質咥仄凶稱辿匹反﹞中內木卞云中化手癆縉蟲厭瞻唱橘及葡難卅芘�互カ戶日木凶‘

卅云﹞呿⻗董坁撙佌洈蔽弇凜﹞pH及庍⑹﹞橾請羈及冂仵允屯五庍祭反﹞中內木及質咥芊卅日太卞魂董迕恟卞云中化手棋說今木卅井勻凶‘

動曉及魂董瑛絆井日﹞呿魂董橢瘀票卞云中化2-(1-丟民伙巨玄平扑)巨正用□伙及民乓奶瓦□朮甩丞旦正□メ陃稱辿卞覆允月癆縉蟲唱橘芘�嶺卞楮仄﹞悻嶺午�爛仄凶‘

卅云﹞呿⻗董坁撙[2-(1-丟民伙巨玄平扑)巨正用□伙]及庍唱葆嶺卞楮允月妐艇扷屢反卅井勻凶‘挀梧蟲匹丐月Isopropyl acetate反汊窖庍唱魂董匹悻嶺午及扷屢互丐勻凶³⁾‘

Ethylene glycol monomethyl ether反メ陃稱辿毛迕中凶gpt唬鰻閡⼳螺庍唱魂董匹阺嶺午及扷屢互丐月互⁴⁾﹞McGregor卞方月伊申亙□匹反汊窖庍唱魂董毛殖戶凶備潘唬鰻匸嶺魂董匹悻嶺午扷屢仄化中月⁵⁾‘引凶﹞仿永玄毛迕中凶戊丟永玄魂董匹反擎錨匹阺嶺﹞彰遼匹悻嶺午及扷屢互丐勻凶⁶⁾‘Ethylene glycol monomethyl ether acetate反汊窖庍唱魂董匹澆阺嶺丐月中反翕阺嶺³⁾﹞扑亦它斥亦它田巨毛迕中凶唱醒嶺腹請魂董匹阺嶺⁷⁾午及扷屢互丐勻凶‘

Ethylene glycol monobutyl ether反汊窖庍唱魂董匹悻嶺³⁾丐月中反阺嶺^{8, 9)}﹞メ陃稱辿毛迕中月鉼呬癆縉坌蟲跟晶魂董匹悻嶺¹⁰⁾﹞メ陃稱辿毛迕中月癆縉蟲唱橘魂董匹悻嶺^{10, 11)}﹞メ陃稱辿毛迕中月唬鰻閡⼳螺庍唱魂董匹阺嶺¹²⁾午及扷屢互丐勻凶‘今日卞﹞穴它旦卅日太卞仿永玄凝傚魂董匹悻嶺¹³⁾午及扷屢互丐勻凶‘著じ佌洈匹及互氏葆嶺魂董卞云中化穴它旦匹阺嶺﹞仿永玄匹翕阺嶺午及瑛絆互丿日木化中月¹³⁾‘

妐艇

1)	Matsuoka, M. Hayashi and M. Ishidate Jr., Mutat. Res., 66, 277(1979).
2)	ざ呿棕雁庍唱葆喀莘’荇し⑹坁魂董坌粗莘彸﹞"祭喀坁撙卞方月癆縉蟲唱橘失玄仿旦﹞"蘋轄踏饕﹞鱗詁﹞1988, pp.31-35.
3)	E. Zeiger, B. Anderson, S. Haworth, T. Lawlor, K. Mortelmans, Environ. Mol. Mutagen, 19(suppl 21), 2(1992).
4)	H. Ma, J. An, A. W. Hsie, W. W. Au, Mutat. Res., 298, 219(1993).
5)	D. McGregor, Occup. Hyg., 2, 213(1996).
6)	D. Anderson, A. Dhawan, T. W. Yu, M. J. Plewa, Mutat. Res., 370, 159(1996).
7)	C. Osgood, S. Zimmering, J. M. Mason, Mutat. Res., 259, 147(1991).
8)	J. C. Hoflack, L. Lambolez, Z. Elias, P. Vasseur, Mutat. Res., 341, 281(1995).
9)	B. B, Gollapudi, E. D. Barber, T. E. Lawlor, et al., Mutat. Res., 370(1), 61(1996).
10)	National Toxicology Program(NTP), NTP No. 26, NIH Publ, No. 93-3349(1993).
11)	Z. Elias, M. C. Daniere, A. M. Marande, O. Poirot, F. Terzetti, O. Schneider, Occup. Hyg., 2, 187(1996).
12)	T. Chiewchanwit, W. W. Au, Mutat. Res., 334, 341(1995).
13)	National Toxicology Program(NTP), NTP TR 484, NIH Draft Publ, No. 98-3974(1998).

洘俀燮

魂董毚エ樊“ 醱髓﹛啎

魂董繫癲樊“ 鼴醱﹛諺﹞ɔ𤚗翮欥﹞腑譚懇睫﹞喔葆敃閡﹞昶枆斗心閡﹞捩唳ɪ戺﹞昶枆舶欥

(算)罹圴ツ啣泔圴兜蟈嶺刉笠本件正□

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Correspondence

Authors: Madoka Nakajima(Study Director)
Jin Tanaka, Shinya Ozaki, Masanori Kikuchi, Reiko Kajihara, Yumiko Suzuki, Miho Nagai, Masaya Suzuki

Biosafety Research Center, Foods, Drugs and Pesticides(An-pyo Center)

582-2 Shioshinden, Fukude-cho, Iwata-gun, Shizuoka, 437-1213, Japan

Tel +81-538-58-1266 Fax +81-538-58-1393

洘俀燮
	魂董毚エ樊“	醱髓﹛啎
	魂董繫癲樊“	鼴醱﹛諺﹞ɔ𤚗翮欥﹞腑譚懇睫﹞喔葆敃閡﹞昶枆斗心閡﹞捩唳ɪ戺﹞昶枆舶欥
	(算)罹圴ツ啣泔圴兜蟈嶺刉笠本件正□
	◣437-1213﹛斃痊萱⺆鼴毀吽鼴蠔梅蕙鼴582-2
	Tel 0538-58-1266	Fax 0538-58-1393

Correspondence
	Authors:	Madoka Nakajima(Study Director) Jin Tanaka, Shinya Ozaki, Masanori Kikuchi, Reiko Kajihara, Yumiko Suzuki, Miho Nagai, Masaya Suzuki
	Biosafety Research Center, Foods, Drugs and Pesticides(An-pyo Center)
	582-2 Shioshinden, Fukude-cho, Iwata-gun, Shizuoka, 437-1213, Japan
	Tel +81-538-58-1266	Fax +81-538-58-1393

2-(1-丟民伙巨玄平扑)巨正用□伙及民乓奶瓦□朮’甩丞旦正□メ陃稱辿毛迕中月癆縉蟲唱橘魂董

In Vitro Chromosomal Aberration Test of 2-(1-Methylethoxy)ethanol in Cultured Chinese Hamster Cells

邰沶

杅芊