2-(1-丟民伙巨玄平扑)巨正用□伙及稱嗜毛迕中月汊窖庍唱魂董

Reverse Mutation Test of 2-(1-Methylethoxy)ethanol in Bacteria

邰沶

2-(1-丟民伙巨玄平扑)巨正用□伙卞勾中化﹞稱嗜毛迕中月汊窖庍唱魂董毛撢雒仄凶‘

魂董嗜堪午仄化﹞Salmonella typhimurium(TA100﹞TA98﹞TA1535﹞TA1537)云方太Escherichia coli(WP2 uvrA)及5嗜堪毛迕中凶‘迕恟澀爛魂董卞云中化反S9 mix拑驕笛歇云方太驕笛歇及備魂董嗜堪卞云中化8.19℅5000 米g/plate及8迕恟﹞呿魂董匹反313℅5000 米g/plate及5迕恟匹魂董毛撢雒仄凶‘

公及瑛絆﹞S9 mix拑驕笛歇云方太驕笛歇及中內木卞云中化手﹞迍モ覆寰卞⺶屯汊窖⼳螺庍唱戊伕瓦□醒及抸割卅餵笛反カ戶日木卅井勻凶‘仇木日徆魂董煙匹及魂董瑛絆反﹞迕恟澀爛魂董云方太呿魂董卞云中化瘋蜇嶺互割カ今木凶‘

動曉及瑛絆方曰﹞呿魂董橢瘀票匹反2-(1-丟民伙巨玄平扑)巨正用□伙反﹞庍唱葆嶺毛肣仄卅中(悻嶺)午瑛狨仄凶‘

杅芊

1.﹛魂董嗜堪

稱嗜毛迕中月汊窖庍唱魂董卞僮仁銀迕今木化中月仇午井日﹞魂董嗜堪午仄化甲旦民斥件邰菲嶺及Salmonella typhimurium TA100﹞TA98﹞TA1535云方太TA1537¹⁾卅日太卞玄伉皿玄白央件邰菲嶺及Escherichia coli WP2 uvrA²⁾及5潘挀及嗜堪毛薊鎗仄凶‘

生朮立民白旦嗜反噯狟58コ9畸9ざ卞市伉白巧伙瓦失釐喀及B. N. Ames項牖井日﹞引凶﹞釐觸嗜卞勾中化反噯狟58コ3畸16ざ卞嶄峏啟戲魂董赭(蜇:嶄峏啣泔圴罹圴啟戲蒂菜赭)井日坌芨毛熬仃凶‘尪嶽12コ10畸27ざ卞嗜堪及冂嶺腹犖毛撢雒仄﹞呿魂董卞迕中凶嗜堪互筋爛及冂嶺毛忡儅仄化中月仇午毛割カ仄凶‘

備嗜堪及嗜睦雙敕反斥丟民伙旦伙石平扑玉(DMSO:MERCK KGaA)毛驕笛仄凶詨﹞鑠瑛忡繡迕民亙□皮卞0.2 mL內勾坌鏜仄凶‘仇木毛敕蟲轎豳毛迕中化鑠瑛仄﹞譯斕硃白伉□扒□卞-80 ∵匹忡繡仄凶‘

2.﹛メ譁及譬擠

1)﹛瘉劑弘伙戊□旦捶韁尪�メ譁(皿伊□玄)

左伉巨件正伙夢抇鼎僅(堪)擠及氾旦丟犯奴失ANメ譁毛壽じ仄﹞魂董卞迕中凶‘呿皿伊□玄反﹞Vogel-Bonner及瘉劑メ譁E毛殖戈踹迍敕(0.02 %帣鄙穴弘生扑它丞’7踹梅﹞0.2 %弁巨件鄙’1踹梅﹞1 %伉件鄙き市伉它丞’拑踹梅﹞0.192 %伉件鄙域失件乒瓦它丞﹞0.066 %踹鄙祭瓜玄伉它丞[中內木手瘉蔽タ蘸])卞2 %及弘伙戊□旦(狟跪賬泔鼎僅(堪))午1.5 %及捶韁(匐〆捶韁BA-30A)毛笛尹﹞溘90 mm及扑乓□伊卞1呦癲凶曰30 mL毛坌鏜仄凶手及匹丐月‘

2)﹛玄永皿失布□(お捶韁)

梅祭瓜玄伉它丞0.5 w/v%毛殖戈0.6 w/v% Bacto-agar(Difco)踹迍敕10芢恟卞覆仄﹞生朮立民白旦嗜毛迕中月魂董及樺寧﹞0.5 mmol/L L-甲旦民斥件(楮鱗祭喀(堪))-0.5 mmol/L D-申左民件(楮鱗祭喀(堪))踹迍敕毛1芢恟笛尹﹞釐觸嗜毛迕中月魂董及樺寧﹞0.5 mmol/L L-玄伉皿玄白央件(楮鱗祭喀(堪))踹迍敕毛⑺元仁1芢恟笛尹迕中凶‘

3.﹛蟆メ陃橢瘀

〇芢恟200 mL及田永白伙尥趕剩白仿旦戊卞2.5 w/v%瓦亙□玄伉巨件玄皮伕旦(Oxoid Nutrient Broth No. 2: OXOID)迍敕毛25 mL坌鏜仄﹞仇木卞芼荸仄凶嗜睦雙敕毛50 米L濤潘仄凶‘它巧□正□田旦扑尼□市□(MM-10:正奶氾永弁(堪))毛迕中﹞37 ∵匹8凜棉蕊熐(欲汊蕊熐:100莢/坌)メ陃仄﹞嗜タ蘸毛割カ仄凶詨魂董卞銀迕仄凶‘

4.﹛S9 mix

擠瞻詨6仰畸動〇及平永戊□穴件(堪)擠S9 mix毛魂董卞銀迕仄凶‘S9 mix醱及S9反芘⑼管午仄化白尼用田伙申正□伙云方太5,6-矛件札白仿示件毛髑芨仄凶Sprague-Dawley煙芮仿永玄及棒癒井日譬擠今木凶手及匹丐月‘S9 mix及賺嶽毛動票卞憎允‘

嶽坌	S9 mix 1 mL醱及恟
S9	0.1 mL
MgCl₂	8 米mol
KCl	33 米mol
G-6-P	5 米mol
NADPH	4 米mol
NADH	4 米mol
Na-伉件鄙森擋敕(pH 7.4)	100 米mol
橈帢踹	酸恟

5.﹛⻗董坁撙

2-(1-丟民伙巨玄平扑)巨正用□伙(伕永玄⺀寞:30321)反賬蘸99.5 %動曉(尕賬坁午仄化踹坌0.1 %動票毛殖戈)及⑨抸敕蟲匹丐月‘呿管反20 ∵及踹云方太橘硃匹及失本玄件卞敦蟈卞迍荸仄﹞踹迍敕醱匹兜爛匹丐月‘趕唳祭喀(堪)(鱗詁)井日羼間今木凶⻗董坁撙毛銀迕仄凶‘⻗董坁撙反﹞銀迕凜引匹撮硃匹忡棟仄凶‘魂董蔽弇詨﹞⻗董坁撙羼間葭卞云中化酸芴⻗董坁撙毛坌橾仄凶瑛絆﹞兜爛嶺卞杽鎖反卅井勻凶‘

6.﹛⻗董坁撙敕及譬擠

魂董及籥蘸﹞⻗董坁撙毛ざ呿泔傾杅鏜槨迕踹((堪)釐騵擠泔鼎樺)毛迕中化迍荸仄化譬擠葆敕午仄凶‘譬擠葆敕毛銀迕迍モ毛迕中化賜慼赭爛タ蘸卞渦澄仄凶詨﹞簧支井卞質咥毛墊勻凶‘

7.﹛魂董迕恟及澀爛

8.19℅5000 米g/plate及8迕恟(跤⺶2.5)毛迕中化迕恟澀爛魂董毛撢雒仄凶‘S9 mix拑驕笛歇云方太驕笛歇及備魂董嗜堪匹中內木及迕恟卞云中化手汊窖⼳螺庍唱戊伕瓦□醒及抸割卅餵笛溯輊反カ戶日木卅井勻凶‘引凶﹞備魂董嗜堪及中內木及質咥歇卞云中化手﹞戲圈賸陴綜迕反棋說今木卅井勻凶‘卅云﹞戊伕瓦□煌醒凜﹞橾請羈及冂仵允屯五庍祭反棋說今木卅井勻凶‘

蝨勻化﹞呿魂董卞云中化反S9 mix拑驕笛歇云方太驕笛歇及備魂董嗜堪卞云中化5000 米g/plate毛瘉嫖迕恟午仄﹞公木冗木5迕恟(跤⺶2)毛澀爛仄凶‘

8.﹛阺嶺覆寰坁撙

阺嶺覆寰坁撙午仄化票筏卞憎仄凶坁撙毛銀迕仄凶‘仇木日及阺嶺覆寰坁撙反DMSO毛迕中化迍荸仄﹞劑恟內勾坌鏜仄凶詨鑠瑛忡繡(-20 ∵)仄凶‘

2-(2-白伉伙)-3-(5-瓦玄伕-2-白伉伙)失弁伉伙失立玉(AF-2:狟跪賬泔鼎僅(堪))

失斥祭瓜玄伉它丞(NaN3:狟跪賬泔鼎僅(堪))

9-失立用失弁伉斥件梅鄙梅(9-AA:Aldrich模)

2-失立用失件玄仿本件(2-AA:狟跪賬泔鼎僅(堪))

9.﹛魂董杅芊

Ames日及葆芊¹⁾及荼恌芊匹丐月皿伊奶件平亙矛□扑亦件芊卞賞元化﹞S9 mix拑驕笛歇云方太驕笛歇公木冗木卞勾中化魂董毛撢雒仄凶‘魂董棟卞﹞銀迕迍モ﹞⻗董坁撙敕丐月中反阺嶺覆寰坁撙迍敕毛100 米L﹞慼中匹S9 mix拑驕笛歇及樺寧﹞0.1 mol/L瓜玄伉它丞’伉件鄙森擋敕(pH 7.4)毛500 米L﹞S9 mix驕笛歇及樺寧﹞S9 mix毛500 米L驕笛仄﹞今日卞魂董嗜敕100 米L毛笛尹﹞37 ∵匹20坌棉蕊熐メ陃(皿伊奶件平亙矛□扑亦件)仄凶‘メ陃蔽弇詨﹞丐日井元戶45 ∵卞忡硃仄凶玄永皿失布□毛2 mL驕笛仄﹞漁寧敕毛皿伊□玄曉卞褐遽仄凶‘37 ∵及橢瘀匹48凜棉備皿伊□玄毛メ陃仄凶詨﹞⻗董坁撙及魂董嗜堪卞覆允月戲圈賸陴綜迕毛割カ允月凶戶﹞撢蟲葡ɵ黃(﹣ 60)毛迕中化皿伊□玄曉及魂董嗜堪及戲圈橇謫毛棋說仄凶‘慼中匹﹞汊窖⼳螺庍唱卞方曰戲元凶戊伕瓦□毛煌醒仄凶‘煌竅卞端仄化反戊伕瓦□失瓜仿奶扒□(CA-11:扑旦氾丞扔奶巨件旦(堪))毛迕中凶‘備タ蘸卞勾五3呦及皿伊□玄毛銀迕仄凶‘

10.﹛瑛絆及荸橾

汊窖⼳螺庍唱戊伕瓦□醒互迍モ覆寰及幻廿2ム動曉卞餵笛仄﹞井勾﹞瘋蜇嶺丐月中反⻗董坁撙及迕恟卞匙繡嶺互カ戶日木凶樺寧卞﹞阺嶺午�爛仄凶‘

卅云﹞讓煌喀讀澎芊毛迕中凶腹爛反撢雒仄卅井勻凶‘

瑛絆云方太嗶說

迕恟澀爛魂董及瑛絆毛Table 1℅2卞﹞呿魂董及瑛絆毛Table 3℅4卞憎仄凶‘S9 mix拑驕笛歇卅日太卞驕笛歇午手中內木及魂董嗜堪卞云中化手﹞2-(1-丟民伙巨玄平扑)巨正用□伙質咥卞方月汊窖⼳螺庍唱戊伕瓦□醒及抸割卅餵笛溯輊反カ戶日木卅井勻凶‘引凶﹞備魂董嗜堪及中內木及質咥歇卞云中化手魂董嗜堪卞覆允月戲圈賸陴綜迕反棋說今木卅井勻凶‘域杅﹞阺嶺覆寰坁撙反公木冗木及魂董嗜堪卞云中化﹞迍モ覆寰歇及2ム動曉及汊窖⼳螺庍唱戊伕瓦□毛芘�仄凶‘卅云﹞戊伕瓦□煌醒凜﹞橾請羈及冂仵允屯五庍祭反棋說今木卅井勻凶‘

動曉及魂董瑛絆井日﹞呿魂董橢瘀票卞云中化﹞2-(1-丟民伙巨玄平扑)巨正用□伙及ɵ戲坁卞覆允月唬鰻閡⼳螺庍唱卞楮仄﹞悻嶺午�爛仄凶‘

卅云﹞呿⻗董坁撙[2-(1-丟民伙巨玄平扑)巨正用□伙]及庍唱葆嶺卞楮允月扷屢反卅井勻凶‘挀梧蟲匹丐月Isopropyl acetate反汊窖庍唱魂董匹悻嶺午及扷屢互丐勻凶³⁾‘

Ethylene glycol monomethyl ether反メ陃稱辿毛迕中凶gpt唬鰻閡⼳螺庍唱魂董匹阺嶺午及扷屢互丐月互⁴⁾﹞McGregor卞方月伊申亙□匹反汊窖庍唱魂董毛殖戶凶備潘唬鰻匸嶺魂董匹悻嶺午扷屢仄化中月⁵⁾‘引凶﹞仿永玄毛迕中凶戊丟永玄魂董匹反擎錨匹阺嶺﹞彰遼匹悻嶺午及扷屢互丐勻凶⁶⁾‘Ethylene glycol monomethyl ether acetate反汊窖庍唱魂董匹澆阺嶺丐月中反翕阺嶺³⁾﹞扑亦它斥亦它田巨毛迕中凶唱醒嶺腹請魂董匹阺嶺⁷⁾午及扷屢互丐勻凶‘

Ethylene glycol monobutyl ether反汊窖庍唱魂董匹悻嶺³⁾丐月中反阺嶺^{8, 9)}﹞メ陃稱辿毛迕中月鉼呬癆縉坌蟲跟晶魂董匹悻嶺¹⁰⁾﹞メ陃稱辿毛迕中月癆縉蟲唱橘魂董匹悻嶺^{10, 11)}﹞メ陃稱辿毛迕中月唬鰻閡⼳螺庍唱魂董匹阺嶺¹²⁾午及扷屢互丐勻凶‘今日卞﹞穴它旦卅日太卞仿永玄凝傚魂董匹悻嶺¹³⁾午及扷屢互丐勻凶‘著じ佌洈匹及互氏葆嶺魂董卞云中化穴它旦匹阺嶺﹞仿永玄匹翕阺嶺午及瑛絆互丿日木化中月¹³⁾‘

妐艇

1)	D. M. Maron and B. N. Ames, Mutat. Res., 113, 173(1983).
2)	M. H. L. Green and W. J. Muriel, Mutat. Res., 38, 3(1976).
3)	E. Zeiger, B. Anderson, S. Haworth, T. Lawlor, K. Mortelmans, Environ. Mol. Mutagen, 19(suppl 21), 2(1992).
4)	H. Ma, J. An, A. W. Hsie, W. W. Au, Mutat. Res., 298, 219(1993).
5)	D. McGregor, Occup. Hyg., 2, 213(1996).
6)	D. Anderson, A. Dhawan, T. W. Yu, M. J. Plewa, Mutat. Res., 370, 159(1996).
7)	C. Osgood, S. Zimmering, J. M. Mason, Mutat. Res., 259, 147(1991).
8)	J. C. Hoflack, L. Lambolez, Z. Elias, P. Vasseur, Mutat. Res., 341, 281(1995).
9)	B. B, Gollapudi, E. D. Barber, T. E. Lawlor, et al., Mutat. Res., 370(1), 61(1996).
10)	National Toxicology Program(NTP), NTP No. 26, NIH Publ, No. 93-3349(1993).
11)	Z. Elias, M. C. Daniere, A. M. Marande, O. Poirot, F. Terzetti, O. Schneider, Occup. Hyg., 2, 187(1996).
12)	T. Chiewchanwit, W. W. Au, Mutat. Res., 334, 341(1995).
13)	National Toxicology Program(NTP), NTP TR 484, NIH Draft Publ, No. 98-3974(1998).

洘俀燮

魂董毚エ樊“ 醱髓﹛啎

魂董繫癲樊“ 鼴醱﹛諺﹞腑譚懇睫﹞氈彌引斗心﹞ɔ𤚗翮欥

(算)罹圴ツ啣泔圴兜蟈嶺刉笠本件正□

◣437-1213﹛斃痊萱⺆鼴毀吽鼴蠔梅蕙鼴582-2

Tel 0538-58-1266 Fax 0538-58-1393

Correspondence

Authors: Madoka Nakajima(Study Director)
Jin Tanaka, Masanori Kikuchi, Mayumi Akahoshi, Shinya Ozaki

Biosafety Research Center, Foods, Drugs and Pesticides(An-pyo Center)

582-2 Shioshinden, Fukude-cho, Iwata-gun, Shizuoka, 437-1213, Japan

Tel +81-538-58-1266 Fax +81-538-58-1393

洘俀燮
	魂董毚エ樊“	醱髓﹛啎
	魂董繫癲樊“	鼴醱﹛諺﹞腑譚懇睫﹞氈彌引斗心﹞ɔ𤚗翮欥
	(算)罹圴ツ啣泔圴兜蟈嶺刉笠本件正□
	◣437-1213﹛斃痊萱⺆鼴毀吽鼴蠔梅蕙鼴582-2
	Tel 0538-58-1266	Fax 0538-58-1393

Correspondence
	Authors:	Madoka Nakajima(Study Director) Jin Tanaka, Masanori Kikuchi, Mayumi Akahoshi, Shinya Ozaki
	Biosafety Research Center, Foods, Drugs and Pesticides(An-pyo Center)
	582-2 Shioshinden, Fukude-cho, Iwata-gun, Shizuoka, 437-1213, Japan
	Tel +81-538-58-1266	Fax +81-538-58-1393