2-Ethylbutyric acid


[CAS No. 88-09-5]

2-Ethylbutanoic acid


Molecular formula: C6H12O2         Molecular weight: 116.16


An in vivo micronucleus test of 2-ethylbutyric acid was performed with bone marrow cells of male BDF1 mice. 2-Ethylbutyric acid did not induce micronuclei in the bone marrow cells.


1. Non-bacterial in vivo test (micronucleus test) 1)

Purity : 99.9 %
Test species/strain : Mouse/BDF1(C57BL/6 × DBA/2)[SPF]
Test method : Guidelines for Screening Mutagenicity Testing of Chemicals (Chemical Substances Control Law of Japan) and
OECD Test Guideline 474
 Route : Oral
 Dosage : 0, 500, 1000, 2000 mg/kg
 Number of animals/group : Males, 5
 Vehicle : Corn oil
 Administration period : Two doses
 Sampling time : 24 hours after the second dose
 Positive control : Mitomycin C
 Tissue : Bone marrow cells
GLP : Yes

 Test results:

No male or female animals died even in the group treated with 2000 mg/kg in the dose-finding study. This chemical did not induce micronucleated erythrocytes in mouse bone marrow cells.
1) The test was performed by the Biosafety Research Center, Foods, Drugs and Pesticides(An-pyo Center), 582-2 Shioshinden, Fukude-cho, Iwata-gun, Shizuoka, 437-1213, Japan. Tel +81-538-58-1266, Fax +81-538-58-1393