Methacrylamide

メタクリルアミド


[CAS No. 79-39-0]

2-Methyl-2-propenamide

2-メチル-2-プロペンアミド

Molecular formula: C4H7NO　Molecular weight: 85.10

ABSTRACT

Methacrylamide was evaluated for reproduction/developmental toxicity in Sprague-Dawley rats at oral doses of 0, 12.5, 50 and 200 mg/kg. Females were administered the test chemical from 14 days before mating through gestation until day 3 of lactation, and males were treated for 42 days from 14 days before mating to necropsy.

With 200 mg/kg, one out of 13 males and four out of 13 females were found dead and one female was sacrificed on becoming moribund. Dragging of hindlimbs appeared in all animals of the 200 mg/kg group. Body weight gain was decreased in the 50 and 200 mg/kg groups. Food consumption was also decreased in males given 50 and 200 mg/kg, and in females receiving 200 mg/kg. Although inflammation of the lung was observed at 200 mg/kg, the reproductive organs were not affected in either sex. Fertility and estrous cyclicity were not altered, but the copulation rate was decreased at 200 mg/kg. Delayed parturition and abnormal nursing were observed at 200 mg/kg. Lower body weights and decreased viability of the pups were observed at 200 mg/kg. No morphological abnormalities were found in any pups.

In conclusion, the NOEL for systemic toxicity was considered to be 12.5 mg/kg/day in male and female rats, and that for reproductive and developmental toxicity was 50 mg/kg/day.

SUMMARIZED DATA FROM THE STUDY

1. Reproductive/Developmental Toxicity 1)

Purity:99 %
Test species/strain:Rat/Crj:CD(SD)IGS
Test method:OECD Test Guideline 421
　Route:Oral(gavage)
　Doses:0(vehicle), 12.5, 50, 200 mg/kg/day
　Number of animals/group:Males, 13; females, 13
　Vehicle:Water for injection
　Administration period:Males, 42 days
Females, from 14 days before mating to day 3 of lactation
　Terminal kill:Males, day 43 of administration
Females, day 4 of lactation
GLP:Yes

　Test results:

At 200 mg/kg, one out of 13 males and four out of 13 females were found dead and one female was sacrificed on becoming moribund. Dragging of hindlimbs appeared in all animals at 200 mg/kg. Body weight gain was decreased at 50 and 200 mg/kg. Food consumption was also decreased in males of the 50 and 200 mg/kg groups, and in females of the 200 mg/kg group. Although a low incidence of pneumonia was observed at 200 mg/kg, the reproductive organs were not affected in either sex. Fertility and estrous cyclicity were not affected, but the copulation rate was decreased at 200 mg/kg. Delayed parturition and abnormal nursing were also observed at 200 mg/kg, along with low body weights and decreased viability of the pups. No morphological abnormalities were found in any pups.

The NOEL for systemic toxicity is considered to be 12.5 mg/kg/day in male and female rats, and that for reproductive and developmental toxicity 50 mg/kg/day.

1)The test was performed by the Hatano Research Institute, Food and Drug Safety Center, 729-5 Ochiai, Hadano, Kanagawa, 257-8523, Japan. Tel +81-463-82-4751　Fax +81-463-82-9627