1,1,2-Trichloroethane

1,1,2-トリクロロエタン

[CAS No. 79-00-5]

Ethane trichloride

エタントリクロリド

Molecular formula: C₂H₃Cl₃　Molecular weight: 133.41

ABSTRACT

An in vivo micronucleus test of 1,1,2-trichloroethane was performed with bone marrow cells of male CD-1 (ICR) mice. 1,1,2-Trichloroethane did not induce micronuclei in bone marrow cells, although it suppressed their proliferation at dose of 400 mg/kg.

SUMMARIZED DATA FROM THE STUDIES

1. Non-bacterial in vivo test (micronucleus test)¹⁾

Purity	:	97.5 %
Test species/strain	:	Mouse/Crj:CD-1 (ICR)
Test method	:	Guidelines for Screening Mutagenicity Testing of Chemicals (Chemincal Substances Control Law of Japan) and OECD Test Guideline 474
Route	:	Oral (gavage)
Dosage	:	0 (vehicle), 100, 200, 400 mg/kg
Number of animals/group	:	Males, 5
Vehicle	:	Olive oil
Administration period	:	Single
Sampling time	:	24 and 48 hours
Positive control	:	Cyclophosphamide
Tissue	:	Bone marrow cells
GLP	:	Yes

　Test results:

The preliminary study had revealed that 1,1,2-trichloroethane induced hypoactivity in mice at doses of 400 mg/kg and above. The toxicity did not substantially vary between males and females. Thus, the micronucleus test was performed in male mice with doses of 400 mg/kg at the highest. This chemical did not induce micronuclei in bone marrow cells up to 400 mg/kg (the maximum tolerated dose), although it suppressed their proliferation at 400 mg/kg.

1) The test was performed by the Hatano Research Institute, Food and Drug Safety Center, 729-5 Ochiai, Hadano-shi, Kanagawa, 257-8523, Japan. Tel +81-463-82-4751 Fax +81-463-82-9627