[CAS No. 79-00-5]

Ethane trichloride


Molecular formula: C2H3Cl3 Molecular weight: 133.41


An in vivo micronucleus test of 1,1,2-trichloroethane was performed with bone marrow cells of male CD-1 (ICR) mice. 1,1,2-Trichloroethane did not induce micronuclei in bone marrow cells, although it suppressed their proliferation at dose of 400 mg/kg.


1. Non-bacterial in vivo test (micronucleus test)1)

Purity:97.5 %
Test species/strain:Mouse/Crj:CD-1 (ICR)
Test method:Guidelines for Screening Mutagenicity Testing of Chemicals (Chemincal Substances Control Law of Japan) and OECD Test Guideline 474
 Route:Oral (gavage)
 Dosage:0 (vehicle), 100, 200, 400 mg/kg
 Number of animals/group:Males, 5
 Vehicle:Olive oil
 Administration period:Single
 Sampling time:24 and 48 hours
 Positive control:Cyclophosphamide
 Tissue:Bone marrow cells

 Test results:

The preliminary study had revealed that 1,1,2-trichloroethane induced hypoactivity in mice at doses of 400 mg/kg and above. The toxicity did not substantially vary between males and females. Thus, the micronucleus test was performed in male mice with doses of 400 mg/kg at the highest. This chemical did not induce micronuclei in bone marrow cells up to 400 mg/kg (the maximum tolerated dose), although it suppressed their proliferation at 400 mg/kg.

1)The test was performed by the Hatano Research Institute, Food and Drug Safety Center, 729-5 Ochiai, Hadano-shi, Kanagawa, 257-8523, Japan. Tel +81-463-82-4751 Fax +81-463-82-9627