Sorbitan monooctadecanoate

ソルビタンモノオクタデカノア－ト

[CAS No. 1338-41-6]

Sorbitan monostearete

ソルビタンモノステアレート

Molecular formula: C24H46O6 Molecular weight: 430.62

ABSTRACT

Sorbitan monooctadecanoate induced structural chromosomal aberrations in CHL/IU cells with an exogeneous metabolic activation system. Polyploidy was also induced without an exogeneous metabolic activation system.

SUMMARIZED DATA FROM THE STUDIES

1. Non-bacterial in vitro test (chromosomal aberration test) 1)

Purity	:	unknown
Type of cell used	:	Chinese hamster lung (CHL/IU) cells
Test method	:	Guidelines for Screening Mutagenicity Testing of Chemicals (Japan)
Solvent	:	0.5% Carboxymethylcellulose sodium solution
Positive controls	:	-S9 mix, Mitomycin C +S9 mix, Cyclophosphamide
Doses	:	-S9 mix (continuous treatment): 0, 0.020, 0.040, 0.080 mg/ml -S9 mix (short-term treatment): 0, 0.13, 0.25, 0.50 mg/ml +S9 mix (short-term treatment): 0,1.1, 2.2, 4.3 mg/ml
S-9	:	Rat liver, induced with phenobarbital and 5,6-benzoflavone
Plates/test	:	2
GLP	:	Yes

　 Test results:

Cytogenetic effects were seen as follows.

At all concentrations (1.1 - 4.3 mg/ml) used for the short-term treatment with metabolic activation, cells with structural chromosomal aberrations (22.0 - 46.5%, including gaps) were induced. At all concentrations (0.13 - 0.50 mg/ml) in the short-term treatment case without metabolic activation, polyploidy (4.50 - 7.00%) was induced.

Lowest concentration producing cytogenetic effects in vitro:

Without metabolic activation (short-term treatment):	0.13 mg/ml (polyploidy)
With metabolic activation (short-term treatment):	1.1 mg/ml (clastogenicity)

Genotoxic effects:

	clastogenicity			polyploidy
	+	?	-	+	?	-
without metabolic activation:	[ ]	[ ]	[*]	[*]	[ ]	[ ]
with metabolic activation:	[*]	[ ]	[ ]	[ ]	[ ]	[*]

1) The tests were performed by the Hatano Research Institute, Food and Drug Safety Center, 729-5 Ochiai, Hadano-shi, Kanagawa, 257, Japan. Tel +81-463-82-4751 Fax +81-463-82-9627