1,1’-Azobisformamide

1,1'-アゾビスホルムアミド


[CAS No. 123-77-3]

Azodicarbonamide

アゾジカルボンアミド

Molecular formula: C2H4N4O2　Molecular weight: 116.08

ABSTRACT

One-generation reproduction toxicity was investigated for 1,1'-azobisformamide by daily oral administration of 0, 100, 300 and 1000 mg/kg to 5 week-old male and to 10 week-old female Sprague-Dawley strain (Crj:CD) rats (25 animals each sex/group). The test substance was given to the males for 10 weeks before mating and thereafter in total for 14 weeks, and to the females for 2 weeks before mating, and throughout the mating, gestation and lactation periods, thus in total 8 weeks. The treatment did not affect their reproductive ability, including the estrous cycle, mating, parturition of the animals and viability, growth and morphology of offspring.

While no adverse effects of 1,1'-azobisformamide were found in males, treatment with 1000 mg/kg to females caused death during the lactation period due to pelvic pyelonephritic kidney lesions.

Under the present conditions, NOELs for reproductive toxicity of 1,1'-azobisformamide are considered to be 1000 mg/kg/day for males and females, and those for toxicity other than reproduction are considered to be 1000 mg/kg/day for males and 300 mg/kg/day for females.

SUMMARIZED DATA OF THE STUDY

1. One-Generation Reproduction Toxicity 1)

Purity:99.5 %
Test species/strain:Rat/Crj:CD(SD)
Test method:OECD Test Guideline 415
　Route:Oral(gavage)
　Doses:0, 100, 300 and 1000 mg/kg
　Number of animals/group:Males, 25; females, 25
　Vehicle:Corn oil
　Administration period:Males, 98 days
Females, from 14 days before mating to day 20 of lactation
　Terminal kill:Males, day 99
Females, day 21 of lactation
Offspring, 21 days after birth
GLP:Yes

　Test results:

While no adverse effects of 1,1'-azobisformamide were found in males, treatment with 1000 mg/kg to females caused death during the lactation period due to pelvic nephritis-like lesions.

No adverse effects on reproductive parameters of parent animals including viability, growth and morphology of offspring were observed.

The NOELs for reproduction are considered to be 1000 mg/kg/day for males and females including offspring. Those for toxicity other than reproduction are considered to be 1000 mg/kg/day for males and 300 mg/kg/day for females.

1)The test was performed by the Hatano Research Institute, Food and Drug Safety Center, 729-5 Ochiai, Hadano-shi, Kanagawa, 257-0025, Japan. Tel +81-463-82-4751 Fax +81-463-82-9627