2-Vinylpyridine

2-ビニルピリジン

[CAS No. 100-69-6]

2-Ethenylpyridine

2-エテニルピリジン

Molecular formula: C7H7N Molecular weight: 105.15

ABSTRACT

2-Vinylpyridine was studied for oral toxicity in rats in 28-day repeat dose toxicity test at doses of 0, 12.5, 50 and 200 mg/kg/day. Salivation was observed in both sexes receiving 50 and 200 mg/kg. Body weight gain was suppressed and food consumption decreased in males receiving 200 mg/kg. Urinalysis revealed decreases in specific gravity in females receiving 50 and 200 mg/kg and volume increase in females receiving 200 mg/kg. Relative testes weights were increased in the males receiving 200 mg/kg. Absolute and relative spleen weights were decreased and relative liver weights increased in females receiving 200 mg/kg. Squamous hyperplasia in the forestomach was observed in the both sexes receiving 50 and 200 mg/kg along with thickening of the mucosa at the higher dose. The NOEL for repeat dose toxicity is considered to be 12.5 mg/kg/day for both sexes.

2-Vinylpyridine was mutagenic to E. coli WP2 uvrA with an exogeneous metabolic activation system.

2-Vinylpyridine induced structural chromosomal aberrations in CHL cells, in the absence or presence of an exogenous metabolic activation system.

SUMMARIZED DATA FROM THE STUDIES

1. Repeat Dose Toxicity１）

Purity	:	98.3%
Test species/strain	:	Rats/Crj: CD (SD)
Test method	:	Guidelines for 28-day Repeat Dose Toxicity Testing of Chemicals (Japan)
Route	:	Oral
Doses	:	0 (vehicle), 12.5, 50, 200 mg/kg/day
Number of animals	:	Males, 5; females,5/group
Vehicle	:	Corn oil
Administration period	:	Males and females, 28 days
Terminal killing	:	Males and females, days 29 or 43
GLP	:	Yes

　Test results:: Salivation was observed in both sexes receiving 50 and 200 mg/kg. Body weight gain was suppressed and food consumption decreased in males receiving 200 mg/kg. Urinalysis revealed decreases in specific gravity in females receiving 50 and 200 mg/kg and volume increase in females receiving 200 mg/kg. Relative testes weights were increased in males receiving 200 mg/kg. Absolute and relative spleen weights were decreased and relative liver weights increased in females receiving 200 mg/kg. Squamous hyperplasia and submucosal edema in the forestomach were observed in both sexes receiving 50 and 200 mg/kg, along with thickening of the mucosa at the higher dose. Moreover, erosion and cellular infiltration in the forestomach were observed in males receiving 200 mg/kg. Submucosal edema and/or erosion in the glandular stomach were also observed in females receiving 50 or 200 mg/kg.; Squamous hyperplasia in the forestomach was still observed in both sexes receiving 200 mg/kg at the end of recovery period, but its incidence and extent were decreased in comparison to those of the same groups at the end of administration period.; The NOEL for repeat dose toxicity is considered to be 12.5 mg/kg/day for both sexes.

2. Genetic Toxicity

2-1. Bacterial test１）

Purity	:	98.3%
Test species/strains	:	S. typhimurium TA100, TA1535, TA98, TA1537, E. coli WP2 uvrA
Test method	:	Guidelines for Screening Mutagenicity Testing of Chemicals (Japan)
Procedures	:	Pre-incubation method
Solvent	:	DMSO
Positive controls	:	-S9 mix; AF-2 (TA100, TA98 and WP2 uvrA), Sodium azide (TA1535) and 9-Aminoacridine (TA1537) +S9 mix; 2-Aminoanthracene (all strains)
Doses	:	-S9 mix; 39.1, 78.1, 156, 313, 625, 1250 and 2500 μg/plate (TA100 and TA1535) 156, 313, 625, 1250, 2500 and 5000 μg/plate (TA98,TA1537 and WP2 uvrA) +S9 mix; 156, 313, 625, 1250, 2500 and 5000 μg/plate +S9 mix (WP2 uvrA; confirmative examination); 2500, 3000, 3500, 4000, 4500 and 5000 μg/plate
S9	:	Rat liver, induced with phenobarbital and 5,6-benzoflavone
Plates/test	:	3
Number of replicates	:	2
GLP	:	Yes

　Test results:

Clear positive mutagenic responses were obtained in E. coli WP2 uvrA with metabolic activation.

Genetic effects:
S. typhimurium TA100, TA1535, TA98 and TA1537

+ ? -

Without metabolic activation: [ ] [ ] [*]

With metabolic activation: [ ] [ ] [*]

E. coli WP2 uvrA

+ ? -

Without metabolic activation: [ ] [ ] [*]

Without metabolic activation: [*] [ ] [ ]

2-2. Non-bacterial in vitro test (chromosomal aberration test)１）

Purity	:	98.3%
Type of cell used	:	Chinese hamster lung (CHL) cells
Test method	:	Guidelines for Screening Mutagenicity Testing of Chemicals (Japan)
Solvent	:	DMSO
Positive controls	:	-S9 mix, Mitomycin C +S9 mix, Cyclophosphamide
Doses	:	-S9 mix (continuous treatment):-S9 mix (24h continuous exposure): 0, 3.75, 7.50, 15.0 μg/ml -S9 mix (48h continuous exposure): 0, 1.88, 3.75, 7.50 μg/ml -S9 mix (short-term exposure): 0, 15.0, 30.3, 60.0 μg/ml +S9 mix (short-term exposure): 0, 37.5, 75.0, 150 μg/ml
S9	:	Rat liver, induced with phenobarbital and 5,6-benzoflavone
Plates/test	:	2
GLP	:	Yes

　Test results:

This chemical induced structural chromosomal aberrations in the absence or presence of an exogenous metabolic activation system.

Lowest concentration producing cytogenetic effects in vitro:

Without metabolic activation (24h treatment): 0.00557 mg/ml (clastogenicity)

Genotoxic effects:

	clastogenicity			polyploidy
	+	?	-	+	?	-
Without metabolic activation:	[*]	[ ]	[ ]	[ ]	[ ]	[*]
With metabolic activation:	[*]	[ ]	[ ]	[ ]	[ ]	[*]

1) The tests were performed by the Biosafety Research Center, Foods, Drugs and Pesticides (An-pyo Center), Japan, 582-2 Shioshinden Arahama, Fukude-cho, Iwata-gun, Shizuoka, 437-12, Japan. Tel +81-538-58-1266 Fax +81-538-58-1393