2,2,4-¥È¥ê¥á¥Á¥ë-1,3-¥Ú¥ó¥¿¥ó¥¸¥ª¡¼¥ë¥¸¥¤¥½¥Ö¥Á¥ì¡¼¥È¤Î¥é¥Ã¥È¤òÍѤ¤¤ë
È¿Éü·Ð¸ýÅêÍ¿ÆÇÀ­¡¦À¸¿£È¯À¸ÆÇÀ­Ê»¹ç»î¸³

Combined Repeat Dose and Reproductive/Developemental Toxicity Screening Test of
2,2,4-Trimetyl-1,3-pentanediol diisobutyrate by Oral Administration in Rats

Í×Ìó

2,2,4-¥È¥ê¥á¥Á¥ë-1,3-¥Ú¥ó¥¿¥ó¥¸¥ª¡¼¥ë¥¸¥¤¥½¥Ö¥Á¥ì¡¼¥È¤Ï±ö²½¥Ó¥Ë¡¼¥ë¤Î²ÄÁººÞ¤È¤·¤Æ»ÈÍѤµ¤ì¤Æ¤¤¤ë²½¹çʪ¤Ç¤¢¤ë¤¬¡¤Ëܲ½¹çʪ¤Î¥Ò¥È¤ä¼Â¸³Æ°Êª¤ÎÀ¸ÂΤ˵ڤܤ¹±Æ¶Á¤Ë¤Ä¤¤¤Æ¤Ï¤Û¤È¤ó¤ÉÃΤé¤ì¤Æ¤¤¤Ê¤¤¡¥º£²ó¡¤2,2,4-¥È¥ê¥á¥Á¥ë-1,3-¥Ú¥ó¥¿¥ó¥¸¥ª¡¼¥ë¥¸¥¤¥½¥Ö¥Á¥ì¡¼¥È¤ÎÆÇÀ­³ØŪÀ­¼Á¤òɾ²Á¤¹¤ë¤¿¤á¤ËÈ¿Éü·Ð¸ýÅêÍ¿ÆÇÀ­¡¦À¸¿£È¯À¸ÆÇÀ­Ê»¹ç»î¸³¤ò¹Ô¤Ã¤¿¡¥¤¹¤Ê¤ï¤Á¡¤2,2,4-¥È¥ê¥á¥Á¥ë-1,3-¥Ú¥ó¥¿¥ó¥¸¥ª¡¼¥ë¥¸¥¤¥½¥Ö¥Á¥ì¡¼¥È¤Î0¡ÊÍÏÇÞÂоȷ²¡Ë¡¤30¡¤150¤ª¤è¤Ó750mg/kg/day¤òSprague-Dawley·Ï¡ÊSlc:SD¡Ë¥é¥Ã¥È¤Î¸òÇÛÁ°2½µ´Ö¤ª¤è¤Ó¸òÇÛ´ü´Ö2½µ´Ö¤òÄ̤¸¤Æ·Ð¸ýÅêÍ¿¤·¡¤¤µ¤é¤Ëͺ¤Ç¤Ï¸òÇÛ´ü´Ö½ªÎ»¸å16Æü´Ö¡¤»ó¤Ç¤ÏÇ¥¿±´ü´Ö¤òÄ̤¸¤ÆʬÊÚ¸å¤ÎÓ®°é3Æü¤Þ¤ÇϢ³ÅêÍ¿¤·¡¤¿Æưʪ¤ËÂФ¹¤ëÈ¿ÉüÅêÍ¿ÆÇÀ­¤ª¤è¤ÓÀ¸¿£Ç½¤Ê¤é¤Ó¤Ë»ùưʪ¤ÎȯÀ¸¡¦È¯°é¤ËµÚ¤Ü¤¹±Æ¶Á¤Ë¤Ä¤¤¤Æ¸¡Æ¤¤·¤¿¡¥

1.È¿ÉüÅêÍ¿ÆÇÀ­

°ìÈ̾õÂ֤ˤÏÈ︳ʪ¼ÁÅêÍ¿¤Î±Æ¶Á¤Ïǧ¤á¤é¤ì¤º¡¤»àË´Îã¤â´Ñ»¡¤µ¤ì¤Ê¤«¤Ã¤¿¡¥ÂνÅÁý²ÃÍÞÀ©¤¬Íº¤Î750mg/kg·²¤Çǧ¤á¤é¤ì¡¤ÀݱÂÎ̤¬»ó¤Î750mg/kg·²¤ÇÁý²Ã¤·¤¿¡¥Íº¤Î·ì±ÕÀ¸²½³ØŪ¸¡ºº¤Ç¤Ï¡¤150¤ª¤è¤Ó750mg/kg·²¤Ç¥¯¥ì¥¢¥Á¥Ë¥ó¤ª¤è¤ÓÁí¥Ó¥ê¥ë¥Ó¥ó¤ÎÁý²Ã¡¤750mg/kg·²¤ÇÁíÃÁÇò¤ÎÁý²Ã¤¬Ç§¤á¤é¤ì¤¿¡¥´ï´±½ÅÎ̤Ïͺ¤Î150¤ª¤è¤Ó750mg/kg·²¤Ç´Î¡½ÅÎ̤¬Áý²Ã¤·¡¤¤Þ¤¿¡¤750mg/kg·²¤Ç¿Õ¡½ÅÎ̤¬Áý²Ã¤·¤¿¡¥»ó¤Ç¤Ï750mg/kg·²¤Ç´Î¡½ÅÎ̤¬Áý²Ã¤·¤¿¡¥ÉÂÍý³Ø¸¡ºº¤Ç¤Ï¡¤Íº¤Î750mg/kg·²¤ÇÆù´ã½ê¸«¤È¤·¤Æ´Î¡¤Î³ì¿§²½¤¬Ç§¤á¤é¤ì¤¿¡¥ÁÈ¿¥³ØŪ¤Ë¤Ï¡¤Íº¤Î150¤ª¤è¤Ó750mg/kg·²¤Ç¿Õ¡¤ÎÇ¢ºÙ´É¾åÈé¤Î¹¥±ö´ðÀ­²½¤ª¤è¤Ó¾Ë»ÒÅ©ÊÑÀ­¤ÎÄøÅÙ¤ÎÁý¶¯¤¬Ç§¤á¤é¤ì¡¤¤µ¤é¤Ë750mg/kg·²¤Ç¶á°ÌÇ¢ºÙ´É¾åÈé¤Î²õ»à¡¤Àþ°Ý²½¡¤±ó°ÌÇ¢ºÙ´É¤Î¹Ð³ÈÄ¥¡¤´Î¡¤Î»éËò½¤Î¸º¾¯¤ª¤è¤Ó¾®ÍÕÃæ¿´À­´ÎºÙ˦¼ðı¤¬Ç§¤á¤é¤ì¤¿¡¥

2.À¸¿£È¯À¸ÆÇÀ­

¸òÈøǽ¡¤¼õÂÛǽ¤ª¤è¤ÓÀ­¼þ´ü´Ñ»¡¤Ç¤Ï¡¤È︳ʪ¼ÁÅêÍ¿¤Î±Æ¶Á¤Ïǧ¤á¤é¤ì¤Ê¤«¤Ã¤¿¡¥Ê¬ÊÚ»þ´Ñ»¡¤Ç¤Ï¡¤Ç¥¿±Æ°Êª¤ÎÁ´Î㤬Àµ¾ï¤ËʬÊÚ¤·Ó®°é´ü´Ö¤òÄ̤¸È︳ʪ¼ÁÅêÍ¿¤Î±Æ¶Á¤Ïǧ¤á¤é¤ì¤Ê¤«¤Ã¤¿¡¥¤Þ¤¿¡¤¿·À¸»ù¤Î³°É½¸¡ºº¤Ç¤âÈ︳ʪ¼ÁÅêÍ¿¤Ë¤è¤ë¤È¹Í¤¨¤é¤ì¤ë°Û¾ï¤Ï¤Ê¤¯¡¤ÂνŤâÓ®°é4Æü¤Þ¤Ç½çÄ´¤ËÁý²Ã¤·¤¿¡¥»à»º»ù¡¤Ó®°é4Æü¤Þ¤Ç¤Î»àË´»ù¤ª¤è¤ÓÓ®°é4Æü¤Î˶¸¡¤Ç¤ÏÈ︳ʪ¼ÁÅêÍ¿¤Ë¤è¤ë¤È¹Í¤¨¤é¤ì¤ë°Û¾ï½ê¸«¤Ïǧ¤á¤é¤ì¤Ê¤«¤Ã¤¿¡¥

°Ê¾å¤Î·ë²Ì¤«¤é¡¤Ëܻ¾ò·ï²¼¤Ë¤ª¤±¤ë2,2,4-¥È¥ê¥á¥Á¥ë-1,3-¥Ú¥ó¥¿¥ó¥¸¥ª¡¼¥ë¥¸¥¤¥½¥Ö¥Á¥ì¡¼¥È¤ÎÈ¿ÉüÅêÍ¿ÆÇÀ­¤Ï¡¤»óͺ¤È¤â¤Ë150mg/kg°Ê¾å¤ÎÅêÍ¿¤Çǧ¤á¤é¤ì¡¤Ìµ±Æ¶ÁÎ̤ϻóͺ¤È¤â¤Ë30mg/kg/day¤ÈȽÃǤµ¤ì¤ë¡¥¤Þ¤¿¡¤»óͺ¤ÎÀ¸¿£¤ËµÚ¤Ü¤¹±Æ¶Á¤ª¤è¤Ó»ùưʪ¤ÎȯÀ¸¡¦È¯°é¤ËµÚ¤Ü¤¹±Æ¶Á¤Ï¡¤750mg/kg/dayÅêÍ¿¤Ë¤è¤Ã¤Æ¤âǧ¤á¤é¤ì¤º¡¤Ìµ±Æ¶ÁÎ̤ϻóͺ¤È¤â¤Ë750mg/kg/day¤ÈȽÃǤµ¤ì¤ë¡¥

ÊýË¡

1.È︳ʪ¼Á

2,2,4-¥È¥ê¥á¥Á¥ë-1,3-¥Ú¥ó¥¿¥ó¥¸¥ª¡¼¥ë¥¸¥¤¥½¥Ö¥Á¥ì¡¼¥È¡Ê2,2,4-trimetyl-1,3-pentanedioldiisobutyrate¡¤ÊÌ̾CS-16¡¤CAS No.6846-50-0¡¤¥Á¥Ã¥½ÀÐÌý²½³Ø(³ô)À½Â¤¡¤Lot.No.803-4603¡¤½ãÅÙ99.7%¡¤Ê¬»ÒÎÌ286.46¡¤Í»ÅÀ-70¡î¡¤Ê¨ÅÀ280¡î¡Ë¤Ï¡¤Ìµ¿§Æ©ÌÀ¤Ê±ÕÂΤǤ¢¤ê¡¤»ÈÍÑ»þ¤Þ¤Ç¼¼²¹¤ÇÌ©ÊÄÊݴɤ·¤¿¡¥

È︳ʪ¼Á¤Ï¡¤¥³¡¼¥ó¥ª¥¤¥ë¡Ê¥Ê¥«¥é¥¤¥Æ¥¹¥¯À½¡Ë¤ËÍϲò¤·¡¤0.6¡¤3¤ª¤è¤Ó15(w/v)¡ó¤ÎÇ»Å٤ˤʤë¤è¤¦³Æ·²¤ÎÅêÍ¿±Õ¤òÄ´À½¤·¤¿¡¥Ä´À½¸å¤Ï¡¤»ÈÍÑ»þ¤Þ¤ÇÎä°Å¾ò·ï²¼¤ÇÌ©ÊÄÊݴɤ·¤¿¡¥Ä´À½±ÕÃæ¤ÎÈ︳ʪ¼Á¤Ï¡¤2(w/v)%Íϱդξì¹çÎä°Å¾ò·ï²¼¤Ç¾¯¤Ê¤¯¤È¤â7Æü´Ö°ÂÄê¤Ç¤¢¤ë¤³¤È¤¬³Îǧ¤µ¤ì¤Æ¤¤¤ë¤¿¤á¡¤Ä´À½¤Ï1½µ´Ö¤Ë1²ó°Ê¾å¤ÎÉÑÅ٤ǹԤ¤¡¤Ä´À½¸å7Æü°ÊÆâ¤Ë»ÈÍѤ·¤¿¡¥

ÅêÍ¿±Õ¤ÎÇ»ÅÙʬÀϤϡ¤Ä´À½³«»Ï»þ¤ËÄ´À½¤·¤¿³Æ·²¤Î¥Ð¥Ã¥Á¤«¤é̵ºî°Ù¤Ë¥µ¥ó¥×¥ë¤òÃê½Ð¤·¼Â»Ü¤·¤¿¡¥¤½¤Î·ë²Ì¡¤94.0¡Á98.0¡ó¤ÎÈϰϤÇÄ´À½¤µ¤ì¤ª¤ê¡¤¤Û¤Ü½êÄêÎ̤Î2,2,4-¥È¥ê¥á¥Á¥ë-1,3-¥Ú¥ó¥¿¥ó¥¸¥ª¡¼¥ë¥¸¥¤¥½¥Ö¥Á¥ì¡¼¥È¤¬´ÞÍ­¤µ¤ì¤Æ¤¤¤¿¤³¤È¤ò³Îǧ¤·¤¿¡¥

2.»ÈÍÑưʪ¤ª¤è¤Ó»ô°é¾ò·ï

»î¸³¤Ë¤Ï¡¤ÆüËÜ¥¨¥¹¥¨¥ë¥·¡¼(³ô)¤«¤é¹ØÆþ¤·¤¿7½µÎð¤ÎSprague-Dawley¡ÊSlc:SD,SPF¡Ë·Ï»óͺ¥é¥Ã¥È¤ò»ÈÍѤ·¤¿¡¥¹ØÆþ¤·¤¿Æ°Êª¤Ï8Æü´Ö¸¡±Ö¡¦Æë²½»ô°é¤·¤¿¸å¡¤°ìÈ̾õÂ֤˰۾郎ǧ¤á¤é¤ì¤Ê¤«¤Ã¤¿¤â¤Î¤ò8½µÎð¤Ç·²Ê¬¤±¤·¤Æ»î¸³¤ËÍѤ¤¤¿¡¥·²Ê¬¤±½ªÎ»»þ¤ÎÂνŤϡ¤Íº¤Ç257¡Á293g¡¤»ó¤Ç168¡Á181g¤ÎÈϰϤǤ¢¤Ã¤¿¡¥

ưʪ¤Ï¡¤²¹ÅÙ22¡Á26¡î¡¤¼¾ÅÙ45¡Á65¡ó¡¤´¹µ¤²ó¿ô15²ó¡¿»þ´Ö¡¤¾ÈÅÙ150¡Á300lux¡¤¾ÈÌÀ»þ´Ö12»þ´Ö¡Ê¸áÁ°7»þÅÀÅô¡¤¸á¸å7»þ¾ÃÅô¡Ë¤ËÀßÄꤵ¤ì¤¿¥Ð¥ê¥¢¥·¥¹¥Æ¥à¤Î»ô°é¼¼¡ÊW5.7¡ßD10.0¡ßH2.5m¡¤142.5m^3)¤Ç»ô°é¤·¤¿¡¥(³ô)Åìµþµ»¸¦¥µ¡¼¥Ó¥¹¤Î¼«Æ°¿åÀö¼°»ô°éµ¡¡ÊW691.0¡ßD79.0¡ßH195.0cm¡Ë¤ò»ÈÍѤ·¡¤¥¢¥ë¥ßÀ½Á°ÌÌ¡¦¾²¥¹¥Æ¥ó¥ì¥¹ÌÖÌÜ»ô°é¥±¡¼¥¸¡ÊW15.8¡ßD23.8¡ßH16.0cm¡¤»ô°é¥±¡¼¥¸¡¦¥¹¥Ú¡¼¥¹6,017cm^3)¤Ëưʪ¤ò1ɤ¤º¤Ä¼ýÍƤ·»ô°é¤·¤¿¡¥Ã¢¤·¡¤¸òÇÛ´ü´ÖÃæ¤Ï¡¤»óͺ¤Îưʪ¤ò¥¢¥ë¥ßÀ½Á°ÌÌ¡¦¾²¥¹¥Æ¥ó¥ì¥¹ÌÖÌÜ»ô°é¥±¡¼¥¸¡ÊW36.8¡ßD25.0¡ßH16.0cm¡¤»ô°é¥±¡¼¥¸¡¦¥¹¥Ú¡¼¥¹14,720cm^3)¤Ë¼ýÍƤ·»ô°é¤·¤¿¡¥Ç¥¿±18Æü°Ê¹ß¤ÎÊìưʪ¤ÏÓ®°é4Æü¤Þ¤Ç¥¢¥ë¥ßÀ½Á°ÌÌ¡¦¾²¥¹¥Æ¥ó¥ì¥¹ÌÖÌÜ»ô°é¥±¡¼¥¸¡ÊW36.8¡ßD25.0¡ßH16.0cm¡Ë¤ËÓ®°é¥È¥ì¡¼¤ª¤è¤ÓÁãºî¤êºàÎÁ¡Ê¥¢¥ë¥Õ¥¡¡¼¥É¥é¥¤¡Ë¤òÆþ¤ì¤Æ»ô°é¤·¤¿¡¥

»ôÎÁ¤Ï¡¤¥ª¥ê¥¨¥ó¥¿¥ë¹ÚÊ칩¶È(³ô)À½Â¤¤ÎNMF¸Ç·¿»ôÎÁ¡ÊÊü¼ÍÀþÌǶݻôÎÁ¡Ë¤ò»ÈÍѤ·¡¤»ô°é´ü´ÖÃ漫ͳ¤ËÀݼ褵¤»¤¿¡¥°û¿å¤Ï¡¤¿åÆ»¿å¤ò¼«Í³¤ËÀݼ褵¤»¤¿¡¥¶¡µë¤·¤¿»ôÎÁ¡¤¿å¤ª¤è¤ÓÁãºî¤êºàÎÁ¤Ë¤Ï»î¸³¤Ë»Ù¾ã¤òÍ褹²ÄǽÀ­¤Î¹Í¤¨¤é¤ì¤ëÔó»¨Êª¤Îº®ºß¤Ï¤Ê¤«¤Ã¤¿¡¥

3.·²Ê¬¤±

»óͺ¤È¤âÅêÍ¿³«»ÏÆü¤ÎÂνŤò¤â¤È¤ËÁØÊ̲½¤·¡¤Ìµºî°ÙÃê½ÐË¡¤Ë¤è¤ê1·²Åö¤¿¤ê12ɤ¤ò¿¶¤êʬ¤±¤¿¡¥·²Ê¬¤±¸å¤Îưʪ¤Î¼±Ê̤ϸÄÂÎÊ̤˼ª¥Ñ¥ó¥Á¤ò¤¹¤ë¤È¤È¤â¤Ë¥±¡¼¥¸¤´¤È¤Ëưʪɸ¼±ÈÖ¹æ(AnimalID-No.)¤ò¤Ä¤±¤¿¡¥

4.ÅêÍ¿ÎÌ¡¤·²¹½À®¡¤ÅêÍ¿´ü´Ö¤ª¤è¤ÓÅêÍ¿ÊýË¡

Ëܻ¤ÎÍÑÎ̤ϡ¤Àè¤Ë¼Â»Ü¤·¤¿¥é¥Ã¥È¤òÍѤ¤¤¿Í½È÷»î¸³¤Î·ë²Ì¤ò»²¹Í¤Ë¤·¤Æ·èÄꤷ¤¿¡¥¤¹¤Ê¤ï¤Á¡¤0¡¤500¡¤750¤ª¤è¤Ó1000mg/kg¤òͺ¤ª¤è¤Ó»ó¤Ë2½µ´ÖϢ³·Ð¸ýÅêÍ¿¤·¤¿·ë²Ì¡¤»óͺ¤Î500mg/kg°Ê¾å¤ÎÅêÍ¿·²¤Ç´Î¡½ÅÎ̤¬Áý²Ã¤·¡¤750mg/kg°Ê¾å¤ÎÅêÍ¿·²¤Ç¿Õ¡¤ª¤è¤ÓÉû¿Õ½ÅÎ̤¬Áý²Ã¤·¤¿¡¥°Ê¾å¤Î·ë²Ì¤ò¤â¤È¤Ë¤ËËܻ¤Î¹âÍÑÎ̤ò750mg/kg¤ËÀßÄꤷ¡¤°Ê²¼¸øÈæ5¤Ë¤Æ½ü¤·¡¤ÃæÍÑÎ̤ò150mg/kg¡¤ÄãÍÑÎ̤ò30mg/kg¤Ë¤½¤ì¤¾¤ìÀßÄꤷ¤¿¡¥

ÅêÍ¿·ÐÏ©¤Ï¡¤OECD¥¬¥¤¥É¥é¥¤¥ó¡ÖÈ¿ÉüÅêÍ¿ÆÇÀ­¡¦À¸¿£È¯À¸ÆÇÀ­Ê»¹ç»î¸³¡×¤Ç»Ø¼¨¤µ¤ì¤Æ¤¤¤ëÅêÍ¿·ÐÏ©¤Ë½à¤¸¤Æ¶¯À©·Ð¸ýÅêÍ¿¤È¤·¤¿¡¥ÅêÍ¿ÍÆÎ̤ϡ¤ÂνÅ100gÅö¤ê0.5ml¤È¤·¡¤¸ÄÂÎÊ̤ˬÄꤷ¤¿ºÇ¿·ÂνŤ˴ð¤Å¤¤¤Æ»»½Ð¤ò¹Ô¤¤¡¤°ß¥¾¥ó¥Ç¤òÍѤ¤¤ÆËèÆü1²ó¡Ê7Æü¡¿½µ¡Ë¶¯À©·Ð¸ýÅêÍ¿¤·¤¿¡¥Âоȷ²¤Ë¤Ï¥³¡¼¥ó¥ª¥¤¥ë¤Î¤ß¤òÅêÍ¿¤·¤¿¡¥

ͺ¤ÎÅêÍ¿´ü´Ö¤Ï¡¤¸òÇÛÁ°14Æü´Ö¤È¸òÇÛ´ü´Ö14Æü´Ö¤ª¤è¤Ó¸òÇÛ´ü´Ö½ªÎ»¸å16Æü´Ö¤ÎϢ³44Æü´Ö¤È¤·¤¿¡¥»ó¤ÎÅêÍ¿´ü´Ö¤Ï¡¤¸òÇÛÁ°14Æü´Ö¤È¸òÇÛ´ü´ÖÃæ¡Ê¸òÈøÀ®Î©¤Þ¤ÇºÇĹ14Æü´Ö¡Ë¤ª¤è¤Ó¸òÈøÀ®Î©»ó¤ÎÇ¥¿±´ü´Ö¤òÄ̤¸¤ÆʬÊÚ¸å¤ÎÓ®°é3Æü¤Þ¤Ç¡Ê40¡Á53Æü´Ö¡Ë¤È¤·¤¿¡¥¤Ê¤ª¡¤¸òÈø¤ÎÀ®Î©¤·¤Ê¤«¤Ã¤¿»ó¤Ï¸òÇÛ´ü´Ö½ªÎ»¸å¤Î²ò˶Á°Æü¤Þ¤Ç44Æü´Ö¤È¤·¡¤¸òÈø¤ÏÀ®Î©¤·¤¿¤¬Ç¥¿±¤¬À®Î©¤·¤Ê¤«¤Ã¤¿»ó¤ÏÇ¥¿±25Æü¤Î²ò˶Á°Æü¤Þ¤Ç¤Î40Æü´Ö¤È¤·¤¿¡¥

5.´Ñ»¡¤ª¤è¤Ó¸¡ºº

1)°ìÈ̾õÂÖ

»óͺ¤È¤â¡¤Á´Îã¤Ë¤Ä¤¤¤Æ»î¸³´ü´ÖÃæËèÆü´Ñ»¡¤·¤¿¡¥

2)ÂνÅ

ͺ¤Ç¤Ï¡¤ÅêÍ¿1¡ÊÅêÍ¿³«»ÏÆü¡Ë¡¤8¡¤15¡¤22, 29¡¤36¡¤43¤ª¤è¤Ó45Æü¡Ê˶¸¡Æü¡Ë¤Ë¬Äꤷ¡¤ÅêÍ¿1¤«¤é43Æü¤Þ¤Ç¤ÎÂνÅÁý²ÃÎ̤ò»»½Ð¤·¤¿¡¥»ó¤Ç¤Ï¡¤ÅêÍ¿1¡ÊÅêÍ¿³«»ÏÆü¡Ë¡¤8¡¤15¤ª¤è¤Ó22Æü¤Ë¬Äꤷ¡¤ÅêÍ¿1¤«¤é15Æü¤Þ¤Ç¤ÎÂνÅÁý²ÃÎ̤ò»»½Ð¤·¤¿¡¥¸òÈø¤ÎÀ®Î©¤·¤Ê¤«¤Ã¤¿»ó¤Ï¤½¤ì°Ê¸å¤ÎÅêÍ¿29¡¤36¡¤43¤ª¤è¤Ó45Æü¤Ë¬Äꤷ¤¿¡¥¤Þ¤¿¡¤¸òÈø¤¬À®Î©¤·¤¿»ó¤Ï¡¤Ç¥¿±0¡¤7¡¤14¤ª¤è¤Ó21Æü¤Ë¡¤Ê¬ÊÚ¤·¤¿»ó¤ÏÓ®°é0¤ª¤è¤Ó4Æü¤Ë¬Äꤷ¡¤¤½¤ì¤¾¤ìÇ¥¿±0¤«¤é21Æü¤ª¤è¤ÓÓ®°é0¤«¤é4Æü¤Þ¤Ç¤ÎÂνÅÁý²ÃÎ̤ò»»½Ð¤·¤¿¡¥

3)ÀݱÂÎÌ

ͺ¤Ç¤Ï¡¤ÅêÍ¿1¡ÊÅêÍ¿³«»ÏÆü¡Ë¡¤8¡¤15¡¤29¡¤36¡¤43¤ª¤è¤Ó44Æü¡Ê˶¸¡Á°Æü¡Ë¤Ë±Â½ÅÎ̤ò¬Äꤷ¡¤Â¬ÄêÆü¤«¤é¼¡¤Î¬ÄêÆü¤Þ¤Ç¤Î´Ö¤ÎÀݱÂÎ̤òµá¤áÊ¿¶Ñ1ÆüÀݱÂÎ̤ò»»½Ð¤¹¤ë¤È¤È¤â¤ËÅêÍ¿1¤«¤é15Æü¤ª¤è¤ÓÅêÍ¿29¤«¤é44Æü¤Þ¤Ç¤ÎÎßÀÑÀݱÂÎ̤ò»»½Ð¤·¤¿¡¥»ó¤Ç¤Ï¡¤ÅêÍ¿1¡ÊÅêÍ¿³«»ÏÆü¡Ë¡¤8¤ª¤è¤Ó15Æü¤Ë¡¤¸òÈø¤ÎÀ®Î©¤·¤Ê¤«¤Ã¤¿»ó¤Ï¤½¤ì°Ê¹ß¤ÎÅêÍ¿29¡¤36¤ª¤è¤Ó43Æü¤Ë±Â½ÅÎ̤ò¬Äꤷ¡¤Â¬ÄêÆü¤«¤é¼¡¤Î¬ÄêÆü¤Þ¤Ç¤ÎÀݱÂÎ̤òµá¤áÊ¿¶Ñ1ÆüÀݱÂÎ̤ò»»½Ð¤¹¤ë¤È¤È¤â¤ËÅêÍ¿1¤«¤é15Æü¤Þ¤Ç¤ÎÎßÀÑÀݱÂÎ̤ò»»½Ð¤·¤¿¡¥¤Þ¤¿¡¤¸òÈøÀ®Î©¤Î»ó¤ÏÇ¥¿±0¡¤7¡¤14¤ª¤è¤Ó21Æü¤Ë¡¤Ê¬ÊÚ¤·¤¿»ó¤ÏÓ®°é0¤ª¤è¤Ó4Æü¤Ë±Â½ÅÎ̤ò¬Äꤷ¡¤Â¬ÄêÆü¤«¤é¼¡¤Î¬ÄêÆü¤Þ¤Ç¤Î´Ö¤ÎÀݱÂÎ̤òµá¤áÊ¿¶Ñ1ÆüÀݱÂÎ̤ò»»½Ð¤¹¤ë¤È¤È¤â¤Ë¤½¤ì¤¾¤ìÇ¥¿±0¤«¤é21Æü¤ª¤è¤ÓÓ®°é0¤«¤é4Æü¤Þ¤Ç¤ÎÎßÀÑÀݱÂÎ̤ò»»½Ð¤·¤¿¡¥¤Ê¤ª¡¤¸òÇÛ´ü´ÖÃæ¤ÎÀݱÂÎ̤ϬÄꤷ¤Ê¤«¤Ã¤¿¡¥

4)¸òÇÛ

¸òÇÛÁ°7Æü´Ö¡Ê¸òÇÛ³«»ÏÆü¤ò´Þ¤á¤Æ8Æü´Ö¡Ë¤ÎÀ­¼þ´ü´Ñ»¡¤ò¹Ô¤Ã¤¿»ó¤òƱ·²Æâ¤Îͺ¤Î¥±¡¼¥¸¤ËÆþ¤ì1ÂÐ1¤ÇºÇĹ2½µ´ÖËèÈÕƱµï¤µ¤»¤¿¡¥ÍâÄ«¡¤çµ¹¤Ãæ¤ÎÀº»Ò³Îǧ¤ò¤â¤Ã¤Æ¸òÈøÀ®Î©¤È¤·¡¤¤½¤ÎÆü¤òÇ¥¿±0Æü¤È¤·¤¿¡¥¸òÇÛ·ë²Ì¤«¤é¡¤³Æ·²¤Ë¤Ä¤¤¤Æ¸òÈøΨ¡Î¡Ê¸òÈøÀ®Î©Æ°Êª¿ô¡¿Æ±µïưʪ¿ô¡Ë¡ß100¡Ï¤ª¤è¤Ó¼õÂÛΨ¡Î¡Ê¼õÂÛưʪ¿ô¡¿¸òÈøÀ®Î©Æ°Êª¿ô¡Ë¡ß100¡Ï¤òµá¤á¤¿¡¥À­¼þ´ü¤Î´Ñ»¡¤Ï¸òÈøÀ®Î©Æü¤Þ¤Ç¹Ô¤¤¡¤È¯¾ð´ü¤«¤é¼¡¤Îȯ¾ð´ü¤Þ¤Ç¤Î´Ö¤ÎÆü¿ô¤òÀ­¼þ´üÆü¿ô¤È¤·Ê¿¶ÑÀ­¼þ´ü¤ò»»½Ð¤·¤¿¡¥

5)¼«Á³Ê¬ÊÚ»þ¤ª¤è¤Ó¿·À¸»ù¤Î´Ñ»¡

¸òÈøÀ®Î©Æ°Êª¤ÏÁ´Îã¤ò¼«Á³Ê¬ÊÚ¤µ¤»¤¿¡¥Ê¬ÊڤγÎǧ¤Ï¸áÁ°Ãæ¡Ê¸áÁ°9¡Á12»þ¡Ë¤Ë¹Ô¤¤¡¤¤³¤Î»þ´ÖÂÓ¤ËʬÊÚ¤¬´°Î»¤·¤Æ¤¤¤ë¤³¤È¤ò³Îǧ¤·¤¿¸ÄÂΤˤĤ¤¤Æ¤½¤ÎÆü¤òÓ®°é0Æü¤È¤·¤¿¡¥¸áÁ°12»þ°Ê¹ß¤ËʬÊÚ¤¬½ªÎ»¤·¤¿¤â¤Î¤Ï¡¤ÍâÆü¤òÓ®°é0Æü¤È¤·¤¿¡¥Ê¬ÊÚ¤ò³Îǧ¤·¤¿Á´Îã¤Ë¤Ä¤¤¤ÆÇ¥¿±´ü´Ö¡ÊÓ®°é0Æü¤Îǯ·îÆü¤«¤éÇ¥¿±0Æü¤Îǯ·îÆü¤ò¸º¤¸¤¿Æü¿ô¡Ë¤ª¤è¤Ó½Ð»ºÎ¨¡Î¡ÊÀ¸»ù½Ð»º»ó¿ô¡¿¼õÂÛ»ó¿ô¡Ë¡ß100¡Ï¤òµá¤á¤¿¡¥

¿·À¸»ù¤ÏÓ®°é0Æü¤Ë½Ð»º»ù¿ô¡ÊÀ¸Â¸»ù¡Ü»àË´»ù¡Ë¤òÄ´¤Ù¡¤Ê¬ÊÚΨ¡Î¡ÊÁí½Ð»º»ù¿ô¡¿Ãå¾²¿ô¡Ë¡ß100¡Ï¤ª¤è¤Ó½ÐÀ¸Î¨¡Î¡Ê½Ð»ºÀ¸»ù¿ô¡¿Áí½Ð»º»ù¿ô¡Ë¡ß100¡Ï¤òµá¤á¤¿¡¥À¸Â¸»ù¤Ë¤Ä¤¤¤Æ¤ÏÀ­Ê̤òȽÄꤹ¤ë¤È¤È¤â¤Ë³°·Á°Û¾ï¤Î̵ͭ¤òÄ´¤Ù¤¿¡¥¤Þ¤¿¡¤Ó®°é0¤ª¤è¤Ó4Æü¤Ë»óͺÊ̤ÎƱʢ»ù½ÅÎ̤ò¬Äꤷ¡¤»óͺÊÌ1ɤÅö¤¿¤ê¤ÎÊ¿¶Ñ½ÅÎ̤ò»»½Ð¤·¤¿¡¥Ó®°é4Æü¤Î¿·À¸»ù¤ÎƱʢ»ù½ÅÎ̤ò¬Äê¸å¤Ë¿·À¸»ùÁ´Îã¤ò¥¨¡Ý¥Æ¥ëËã¿ì¤Ë¤è¤êÅË»¦¤·¡¤¼çÍ×´ï´±¤ÎÆù´ã´Ñ»¡¤ò¹Ô¤Ã¤¿¡¥¤Ê¤ª¡¤Ó®°é´ü´ÖÃæ¤Î»àË´»ù¤Ë¤Ä¤¤¤Æ¤âƱÍͤ˼çÍ×´ï´±¤ÎÆù´ã´Ñ»¡¤ò¹Ô¤Ã¤¿¡¥¤Þ¤¿¡¤¿·À¸»ù¤Î4ÆüÀ¸Â¸Î¨¡Î¡ÊÓ®°é4ÆüÀ¸»ù¿ô¡¿½Ð»ºÀ¸»ù¿ô¡Ë¡ß100¡Ï¤òµá¤á¤¿¡¥

6)Î×¾²¸¡ºº

³Æ·²¤ÎͺÁ´Îã¤Ë¤Ä¤¤¤Æ˶¸¡»þ¤Ë¼Â»Ü¤·¤¿¡¥Æ°Êª¤òÌó16»þ´ÖÀä¿©¤µ¤»¤¿¸å¡¤¥¨¡¼¥Æ¥ëËã¿ì²¼¤Ç³«Ê¢¤·¡¤Ê¢ÉôÂçÆ°Ì®¤«¤éºÎ·ì¤·¤¿¡¥

a)·ì±Õ³ØŪ¸¡ºº

¸¡ºº¤ÏEDTA-3K¤òź²Ã¤·¤¿½é·ì¤Ë¤Ä¤¤¤Æ¡¤THMS H 6000¡Ê¥Æ¥¯¥Ë¥³¥ó¼Ò¡Ë¤òÍѤ¤¤ÆÇò·ìµå¿ô¡ÊWBC¡§°Å»ëÌîÈÄË¡¡Ë¡¤ÀÖ·ìµå¿ô¡ÊRBC¡§°Å»ëÌîÈÄË¡¡Ë¡¤¥Ø¥Þ¥È¥¯¥ê¥Ã¥ÈÃÍ¡ÊHCT¡§Á´ÀÖ·ìµå¤ÎÍÆÀѤè¤êÊäÀµ¡Ë¡¤¥Ø¥â¥°¥í¥Ó¥óÎÌ¡ÊHGB¡§¥·¥¢¥ó¥á¥È¥Ø¥â¥°¥í¥Ó¥óË¡¡Ë¡¤Ê¿¶ÑÀÖ·ìµåÍÆÀÑ¡ÊMCV¡§RBC, HCT¤è¤ê»»½Ð¡Ë¡¤Ê¿¶ÑÀÖ·ìµå·ì¿§ÁÇÎÌ¡ÊMCH¡§HGB, RBC¤è¤ê»»½Ð¡Ë¡¤Ê¿¶ÑÀÖ·ìµå·ì¿§ÁÇÇ»ÅÙ¡ÊMCHC¡§HGB, HCT¤è¤ê»»½Ð¡Ë¡¤·ì¾®ÈÄ¿ô¡ÊPLT¡§°Å»ëÌîÈÄË¡¡Ë¤ª¤è¤ÓÇò·ìµåɴʬΨ¡Ê¥Õ¥í¡¼¥µ¥¤¥È¥±¥ß¥¹¥È¥ê¡¼Ë¡¡Ë¤ò¬Äꤷ¤¿¡¥

b)·ì±ÕÀ¸²½³ØŪ¸¡ºº

¸¡ºº¤Ï¥¯¥ê¡¼¥ó¥·¡¼¥ë¡Ê(³ô)¥ä¥È¥í¥ó¡Ë¤Ë·ì±Õ¤òºÎ¼è¤·¡¤30ʬ´ÖÊüÃÖ¸å3,000r.p.m. 7ʬ´Ö±ó¿´Ê¬Î¥¤·¤ÆÆÀ¤¿·ìÀ¶¤Ë¤Ä¤¤¤Æ¡¤Â¿¹àÌÜÀ¸²½³Ø¼«Æ°Ê¬ÀÏÁõÃÖCentrifiChem ENCORE ­¶¡Ê¥Ù¡¼¥«¡¼¼Ò¡Ë¤ª¤è¤Ó EKTACHEM 700N¡Ê¥³¥À¥Ã¥¯¼Ò¡Ë¤òÍѤ¤¤ÆÁíÃÁÇò¡Ê¥Ó¥å¡¼¥ì¥Ã¥ÈË¡¡Ë¡¤¥¢¥ë¥Ö¥ß¥ó¡ÊB.C.G.Ë¡¡Ë¡¤A/G¡Ê·×»»ÃÍ¡Ë¡¤·ìÅü¡Ê¥°¥ë¥³¡¼¥¹¥ª¥­¥·¥À¡¼¥¼Ë¡¡Ë¡¤Ç¢ÁÇÃâÁÇ¡Ê¥¦¥ì¥¢¡¼¥¼²þÎÉË¡¡Ë¡¤¥¯¥ì¥¢¥Á¥Ë¥ó¡ÊJaffË¡¡Ë¡¤Áí¥Ó¥ê¥ë¥Ó¥ó¡Ê¥¸¥¢¥¾¿§ÁÇË¡¡Ë¡¤¥°¥ë¥¿¥ß¥ó»À¥ª¥­¥¶¥í¿Ý»À¥È¥é¥ó¥¹¥¢¥ß¥Ê¡¼¥¼¡ÊKarmen²þÎÉË¡¡Ë¡¤¥°¥ë¥¿¥ß¥ó»À¥Ô¥ë¥Ó¥ó»À¥È¥é¥ó¥¹¥¢¥ß¥Ê¡¼¥¼¡ÊKarmen²þÎÉË¡¡Ë¡¤¦Ã¡¼¥°¥ë¥¿¥ß¥ë¥È¥é¥ó¥¹¥Ú¥×¥Á¥À¡¼¥¼¡ÊSzasz²þË¡¡Ë¡¤¥«¥ê¥¦¥à¡ÊÅŶËË¡¡Ë¡¤±öÁÇ¡ÊÅŶËË¡¡Ë¡¤¥«¥ë¥·¥¦¥à¡Ê¥¢¥ë¥»¥Ê¥¾­·¿§ÁÇË¡¡Ë¤ª¤è¤Ó̵µ¡¥ê¥ó¡Ê¥â¥ê¥Ö¥Ç¥ó»À¥¢¥ó¥â¥Ë¥¦¥àË¡¡Ë¤ò¬Äꤷ¤¿¡¥

6.ÉÂÍý³Ø¸¡ºº

1)˶¸¡¤ª¤è¤Ó´ï´±½ÅÎÌ

a)ͺưʪ

44Æü´ÖÅêÍ¿¤·¤¿Æü¤ÎͼÊý¤«¤é±Â¤ò½ü¤­¡¤Ìó16»þ´Ö¤ÎÀä¿©¤µ¤»¤¿ÍâÆü¤Ë¥¨¡Ý¥Æ¥ëËã¿ì²¼¤ÇºÎ·ì¸å°Â³Ú»à¤µ¤»¤¿¡¥Ë¶¸¡¤Ç¤Ï¼çÍ×´ï´±¤ÎÆù´ãŪ´Ñ»¡¤ò¹Ô¤¤¡¤¶»Á£¡¤´Î¡¡¤¿Õ¡¡¤ÀºÁ㤪¤è¤ÓÀºÁã¾åÂνÅÎ̤ò¬Äꤷ´ï´±½ÅÎÌ¡¦ÂνÅÈæ¡ÊÁêÂнÅÎ̡ˤòµá¤á¤¿¡¥¤Þ¤¿¡¤Á´Æ°Êª¤Î½ÅÎ̬Äê´ï´±¤Ë²Ã¤¨¤ÆǾ¡¤¿´Â¡¡¤ç£Â¡¡¤Éû¿Õ¡¤ÀºÇ¹¡¤Á°Î©Á£¡¤²¼¿âÂΤª¤è¤ÓÆù´ã½ê¸«¤È¤·¤ÆÊѲ½¤¬Ç§¤á¤é¤ì¤¿´ï´±¡¦ÁÈ¿¥¤È¤·¤ÆÇÙ¤ª¤è¤ÓÈé²¼Éô¤Î¼ðáî¤ò10¡óÃæÀ­´Ë¾×¥Û¥ë¥Þ¥ê¥ó±Õ¤Ç¸ÇÄꤷ¤¿¡¥¤Ê¤ª¡¤ÀºÁ㤪¤è¤ÓÀºÁã¾åÂΤϥ֥¢¥ó»á±Õ¤Ç¸ÇÄꤷ¤¿¡¥

b)¼«Á³Ê¬ÊÚ¤·¤¿»ó

Ó®°é4Æü¤Ë¥¨¡¼¥Æ¥ëËã¿ì²¼¤ÇÊü·ì°Â³Ú»à¤µ¤»¤¿¡¥Ë¶¸¡¤Ç¤Ï¼çÍ×´ï´±¤ÎÆù´ãŪ´Ñ»¡¤ò¹Ô¤¤¡¤¶»Á£¡¤´Î¡¡¤¿Õ¡¤ª¤è¤ÓÍñÁã½ÅÎ̤ò¬Äꤷ´ï´±½ÅÎÌ¡¦ÂνÅÈæ¡ÊÁêÂнÅÎ̡ˤòµá¤á¤¿¡¥¤Þ¤¿¡¤Á´Æ°Êª¤Î½ÅÎ̬Äê´ï´±¤Ë²Ã¤¨¤ÆǾ¡¤¿´Â¡¡¤ç£Â¡¡¤Éû¿Õ¡¤²¼¿âÂΤª¤è¤ÓÆù´ã½ê¸«¤È¤·¤ÆÊѲ½¤¬Ç§¤á¤é¤ì¤¿´ï´±¡¦ÁÈ¿¥¤È¤·¤ÆÇÙ¤ò10¡óÃæÀ­´Ë¾×¥Û¥ë¥Þ¥ê¥ó±Õ¤Ç¸ÇÄꤷ¤¿¡¥Ë¶¸¡»þ¤Ë²«Âοô¤ª¤è¤ÓÃå¾²¿ô¤òÄ´¤Ù¡¤Ã徲Ψ¡Î¡ÊÃå¾²¿ô¡¿Ç¥¿±²«Âοô¡Ë¡ß100¡Ï¤òµá¤á¤¿¡¥

c)¸òÈøÉÔÀ®Î©¤Î»ó

44Æü´ÖÅêÍ¿¤·¤¿ÍâÆü¤Ë¡¤¥¨¡¼¥Æ¥ëËã¿ì²¼¤ÇÊü·ì°Â³Ú»à¤µ¤»¤¿¡¥Ë¶¸¡¤Ç¤Ï¼çÍ×´ï´±¤ÎÆù´ãŪ´Ñ»¡¤ò¹Ô¤¤¡¤ÈéÉ桤ÆýÁ£¡¤¥ê¥ó¥ÑÀᡤÂñÕÁ£¡¤¶»¹ü¡¤ÂçÂܹü¡Ê¹ü¿ñ¤ò´Þ¤à¡Ë¡¤¶»Á£¡¤µ¤´É¡¤ÇÙ¤ª¤è¤Óµ¤´É»Ù¡¤¿´Â¡¡¤¹Ã¾õÁ£¤ª¤è¤Ó¾åÈé¾®ÂΡ¤À塤¿©Æ»¡¤°ß¡¤½½Æó»ØIJ¡¤¾®Ä²¡¤ÂçIJ¡¤´Î¡¡¤ç¹Â¡¡¤ç£Â¡¡¤¿Õ¡¡¤Éû¿Õ¡¤ç¯æù¡¤ÍñÁ㡤»ÒµÜ¡¤çµ¡¤´ãµå¡¤¥Ï¡¼¥À¡¼Á£¡¤Ç¾¡¤²¼¿âÂΤª¤è¤ÓÀÔ¿ñ¤ò10¡óÃæÀ­´Ë¾×¥Û¥ë¥Þ¥ê¥ó±Õ¤Ç¸ÇÄꤷ¤¿¡¥

d)¼«Á³Ê¬ÊÚ¤Îǧ¤á¤é¤ì¤Ê¤¤»ó

Ç¥¿±25Æü¤Ë¡¤¥¨¡¼¥Æ¥ëËã¿ì²¼¤ÇÊü·ì°Â³Ú»à¤µ¤»¤¿¡¥Ë¶¸¡¤Ç¤Ï¼çÍ×´ï´±¤ÎÆù´ãŪ´Ñ»¡¤ò¹Ô¤¤¡¤ÈéÉ桤ÆýÁ£¡¤¥ê¥ó¥ÑÀᡤÂñÕÁ£¡¤¶»¹ü¡¤ÂçÂܹü¡Ê¹ü¿ñ¤ò´Þ¤à¡Ë¡¤¶»Á£¡¤µ¤´É¡¤ÇÙ¤ª¤è¤Óµ¤´É»Ù¡¤¿´Â¡¡¤¹Ã¾õÁ£¤ª¤è¤Ó¾åÈé¾®ÂΡ¤À塤¿©Æ»¡¤°ß¡¤½½Æó»ØIJ¡¤¾®Ä²¡¤ÂçIJ¡¤´Î¡¡¤ç¹Â¡¡¤ç£Â¡¡¤¿Õ¡¡¤Éû¿Õ¡¤ç¯æù¡¤ÍñÁ㡤»ÒµÜ¡¤çµ¡¤´ãµå¡¤¥Ï¡¼¥À¡¼Á£¡¤Ç¾¡¤²¼¿âÂΤª¤è¤ÓÀÔ¿ñ¤ò10¡óÃæÀ­´Ë¾×¥Û¥ë¥Þ¥ê¥ó±Õ¤Ç¸ÇÄꤷ¤¿¡¥Ãå¾²º¯¤¬Ç§¤á¤é¤ì¤Ê¤¤Æ°Êª¤ÏÇ¥¿±ÉÔÀ®Î©¤ÈȽÄꤷ¤¿¡¥

2)ÉÂÍýÁÈ¿¥³Ø¸¡ºº

a)Ç¥¿±¤òÀ®Î©¤µ¤»¤¿Íº

Âоȷ²¤È¹âÍÑÎÌ·²Á´Îã¤ÎǾ¡¤¶»Á£¡¤¿´Â¡¡¤´Î¡¡¤¿Õ¡¡¤ç£Â¡¡¤Éû¿Õ¡¤ÀºÁ㤪¤è¤ÓÉÂÊÑÉôÁÈ¿¥¤È¤·¤ÆÇÙ¤ª¤è¤ÓÈé²¼Éô¤Î¼ðáî¤Ë¤Ä¤¤¤Æ¼Â»Ü¤·¤¿¡¥¤Ê¤ª¡¤150mg/kg°Ê¾å¤ÎÅêÍ¿·²¤Ç´Î¡½ÅÎ̤¬¡¤750mg/kg·²¤Ç´Î¡½ÅÎ̤¬¤½¤ì¤¾¤ìÁý²Ã¤·¤¿¤¿¤á¡¤30¤ª¤è¤Ó150mg/kg·²¤ÎÁ´Îã¤Î´Î¡¤ª¤è¤Ó¿Õ¡¤Ë¤Ä¤¤¤Æ¤âÁÈ¿¥³Ø¸¡ºº¤ò¼Â»Ü¤·¤¿¡¥

b)¼«Á³Ê¬ÊÚ¤·¤¿»ó

Âоȷ²¤È¹âÍÑÎÌ·²Á´Îã¤ÎǾ¡¤¶»Á£¡¤¿´Â¡¡¤´Î¡¡¤¿Õ¡¡¤ç£Â¡¡¤Éû¿Õ¡¤ÍñÁ㤪¤è¤ÓÉÂÊÑÉôÁÈ¿¥¤È¤·¤ÆÇ٤ˤĤ¤¤Æ¼Â»Ü¤·¤¿¡¥¤Ê¤ª¡¤750mg/kg·²¤Ç´Î¡½ÅÎ̤¬Áý²Ã¤·¡¤Íº¤Ç¿Õ¡½ÅÎ̤ÎÁý²Ã¤¬Ç§¤á¤é¤ì¤¿¤¿¤á¡¤30¤ª¤è¤Ó150mg/kg·²¤ÎÁ´Îã¤Î´Î¡¤ª¤è¤Ó¿Õ¡¤Ë¤Ä¤¤¤Æ¤âÁÈ¿¥³Ø¸¡ºº¤ò¼Â»Ü¤·¤¿¡¥

c)¸òÈø¤ÎÀ®Î©¤·¤Ê¤«¤Ã¤¿»óͺ

Á´Îã¤ÎǾ¡¤¶»Á£¡¤¿´Â¡¡¤´Î¡¡¤¿Õ¡¡¤ç£Â¡¡¤Éû¿Õ¡¤çµ¡¤»ÒµÜ¡¤ÍñÁ㡤ÀºÁ㡤ÀºÁã¾åÂΡ¤ÀºÇ¹¡¤Á°Î©Á£¤ª¤è¤Ó²¼¿âÂΤˤĤ¤¤Æ¼Â»Ü¤·¤¿¡¥

d)Ç¥¿±¤òÀ®Î©¤µ¤»¤Ê¤«¤Ã¤¿Íº¤ª¤è¤ÓÇ¥¿±ÉÔÀ®Î©¤Î»ó

Á´Îã¤ÎǾ¡¤¶»Á£¡¤¿´Â¡¡¤´Î¡¡¤¿Õ¡¡¤ç£Â¡¡¤Éû¿Õ¡¤çµ¡¤»ÒµÜ¡¤ÍñÁ㡤ÀºÁ㡤ÀºÁã¾åÂΡ¤ÀºÇ¹¡¤Á°Î©Á£¤ª¤è¤Ó²¼¿âÂΤˤĤ¤¤Æ¼Â»Ü¤·¤¿¡¥

7.Åý·×²òÀÏ

Âνš¤ÀݱÂÎÌ¡¤²«Âοô¡¤Ãå¾²º¯¿ô¡¤½Ð»º»ù¿ô¡¤À­È桤ʿ¶ÑÀ­¼þ´ü¡¤Ç¥¿±´ü´Ö¡¤Ã徲Ψ¡¤Ê¬ÊÚΨ¡¤½ÐÀ¸Î¨¡¤³°·Á°Û¾ïȯ¸½Î¨¡¤¿·À¸»ù¤Î4ÆüÀ¸Â¸Î¨¡¤´ï´±½ÅÎÌ¡¤´ï´±½ÅÎÌ¡¦ÂνÅÈæ¡ÊÁêÂнÅÎÌ¡Ë¡¤·ì±Õ³ØŪ¤ª¤è¤Ó·ì±Õ²½³ØŪ¸¡ººÃͤˤĤ¤¤Æ¤Ï¤Þ¤ºBartlett¤ÎÅùʬ»¶¸¡Äê2)¤ò¼Â»Ü¤·¤¿¡¥Åùʬ»¶¤Î¾ì¹ç¤Ï°ì¸µÇÛÃÖʬ»¶Ê¬ÀϤò¹Ô¤Ã¤¿¡¥Ê¬»¶¤¬Í­°Õ¤Ç³Æ·²¤ÎɸËÜ¿ô¤¬Æ±¿ô¤Î¾ì¹ç¤ÏDunnett¤Î¿½ÅÈæ³Ó¸¡Äꡤ³Æ·²¤ÎɸËÜ¿ô¤¬°Û¤Ê¤ë¾ì¹ç¤ÏScheff¤Î¿½ÅÈæ³Ó¸¡Äê¤ÇÂоȷ²¤È³ÆÅêÍ¿·²´Ö¤ÎÍ­°Õº¹¤ò¸¡Äꤷ¤¿¡¥Bartlett¤ÎÅùʬ»¶¸¡Äê¤ÇÉÔÅùʬ»¶¤Î¾ì¹ç¤ÏKruskal-Wallis¤Î½ç°Ì¸¡Äê¤ò¼Â»Ü¤·¤¿¡¥Í­°Õ¤Ç³Æ·²¤ÎɸËÜ¿ô¤¬Æ±¿ô¤Î¾ì¹ç¤ÏDunnett¤Î½ç°Ì¸¡Äꡤ³Æ·²¤ÎɸËÜ¿ô¤¬°Û¤Ê¤ë¾ì¹ç¤ÏScheff¤Î½ç°Ì¸¡Äê¤ÇÂоȷ²¤È³ÆÅêÍ¿·²´Ö¤ÎÍ­°Õº¹¤ò¸¡Äꤷ¤¿¡¥½Ð»ºÎ¨¡¤¸òÈøΨ¤ª¤è¤Ó¼õÂÛΨ¤Ë¤Ä¤¤¤Æ¤Ï¦Ö^2¸¡Äê3) 4)¤òÍѤ¤¤¿¡¥ÉÂÍý³Ø¸¡ºº¤Î°Û¾ï½ê¸«ÉÑÅ٤ˤĤ¤¤Æ¤ÏFisher¤ÎľÀܳÎΨ¸¡ÄêË¡4)¤òÍѤ¤¤¿¡¥Ã¢¤·¡¤ÉÂÍýÁÈ¿¥³Ø¸¡ºº¤Î´Î¡¤ª¤è¤Ó¿Õ¡¤Çǧ¤á¤é¤ì¤¿½ê¸«¤Ë¤Ä¤¤¤Æ¤ÏÎßÀÑ¥«¥¤¼«¾è¸¡Äꣴ¡Ë¤ò¼Â»Ü¤·¤¿¡¥¤Ê¤ª¡¤Ó®°é´ü´ÖÃæ¤Î¿·À¸»ù¤Ë´Ø¤¹¤ëÀ®ÀÓ¤Ï1ÊìÂÎÅö¤ê¤ÎÊ¿¶Ñ¤ò1ɸËܤȤ·¤¿¡¥Í­°Õ¿å½à¤Ï*¡§P<0.05 ¤ª¤è¤Ó**¡§P<0.01¤Î2Ãʳ¬¤È¤·¤¿¡¥

·ë²Ì

1.È¿ÉüÅêÍ¿ÆÇÀ­

1)»àË´¤ª¤è¤Ó°ìÈ̾õÂÖ

»àË´Îã¤Ï¡¤ÅêÍ¿´ü´Ö¤òÄ̤¸»óͺ¤¤¤º¤ì¤Î·²¤Ë¤â´Ñ»¡¤µ¤ì¤Ê¤«¤Ã¤¿¡¥°ìÈ̾õÂ֤δѻ¡¤Ç¤Ï¡¤ÇØÉôÈé²¼¤Î¼ðáî¡Ê5mmÂç¡Ë¤¬Íº¤Î750mg/kg·²¤ÇÅêÍ¿6¤«¤é7½µ¤Ë1Îã¤Ë´Ñ»¡¤µ¤ì¤¿¡¥»ó¤Ç¤Ï¡¤´ãʬÈçʪ¤¬Âоȷ²¤ÇÓ®°é3Æü¤Ë¡¤30mg/kg·²¤ÇÓ®°é2¤«¤é4Æü¤Ë¡¤750mg/kg·²¤ÇÇ¥¿±2¤«¤é5Æü¤ª¤è¤Ó13Æü¤Ë¤½¤ì¤¾¤ì1Îã¤Ë´Ñ»¡¤µ¤ì¤¿¡¥

2)ÂνšÊFig. 1, 2¡Ë

ͺ¤Ç¤Ï¡¤750mg/kg·²¤ÇÂоȷ²¤ËÈæ¤Ù¤ÆÅêÍ¿´ü´Ö¤òÄ̤¸ÂνŤ¬¸º¾¯·¹¸þ¤ò¼¨¤·¡¤ÅêÍ¿1¤«¤é43Æü¤ÎÂνÅÁý²ÃÎ̤⸺¾¯¤·¤¿¡¥»ó¤Ç¤Ï¡¤³Æ·²¤ÎÂνŤÏƱÍͤÊÁý²Ã¿ä°Ü¤ò¼¨¤·¡¤Âоȷ²¤ÈÈ︳ʪ¼ÁÅêÍ¿·²¤È¤Î´Ö¤Çº¹¤Ïǧ¤á¤é¤ì¤Ê¤«¤Ã¤¿¡¥

3)ÀݱÂÎÌ¡ÊFig. 3, 4¡Ë

ͺ¤Ç¤Ï¡¤Âоȷ²¤ÈÅêÍ¿·²¤È¤Î´Ö¤Ëº¹¤Ïǧ¤á¤é¤ì¤Ê¤«¤Ã¤¿¡¥»ó¤Ç¤Ï¡¤750mg/kg·²¤ÇÇ¥¿±0¤«¤é7Æü¤ª¤è¤Ó7¤«¤é14Æü¤Î´Ö¡¤Âоȷ²¤ËÈæ¤Ù¤ÆÀݱÂÎ̤¬Áý²Ã¤·¡¤Ç¥¿±0¤«¤é21Æü¤ÎÎßÀÑÀݱÂÎ̤âÂоȷ²¤ËÈæ¤Ù¤ÆÁý²Ã¤·¤¿¡¥

4)ͺ¤Î·ì±Õ³ØŪ¸¡ºº

Çò·ìµå¿ô¤ÏÂоȷ²¤ÈÈ︳ʪ¼ÁÅêÍ¿·²¤È¤Î´Ö¤Ëº¹¤Ï¤Ê¤«¤Ã¤¿¡¥¤¹¤Ù¤Æ¤ÎÅêÍ¿·²¤ÇÂоȷ²¤ËÈæ¤Ù¹¥ÃæµåÈæΨ¤Î¸º¾¯¤ª¤è¤Ó¥ê¥ó¥ÑµåÈæΨ¤ÎÁý²Ã¤¬Ç§¤á¤é¤ì¤¿¤¬¡¤Âоȷ²¤ÎÊÑÆ°¤ÎÈÏ°ÏÆâ¡ÊÂоȷ²¤Î2ɸ½àÊк¹¤ÎÈϰϡˤǤ¢¤Ã¤¿¡¥ÀÖ·ìµå¿ô¤ÏÂоȷ²¤ÈÈ︳ʪ¼ÁÅêÍ¿·²¤È¤Î´Ö¤Ëº¹¤Ï¤Ê¤«¤Ã¤¿¡¥30¤ª¤è¤Ó150mg/kg·²¤ÇÂоȷ²¤ËÈæ¤ÙÊ¿¶ÑÀÖ·ìµåÍÆÀѤ¬¶Ï¤«¤Ë¸º¾¯¤·¤¿¤¬¡¤ÍÑÎ̤˴ØÏ¢¤·¤¿ÊѲ½¤Ç¤Ï¤Ê¤«¤Ã¤¿¡¥¤Ê¤ª¡¤³ÆÅêÍ¿·²¤È¤âÉÏ·ì·¹¸þ¤¬Ç§¤á¤é¤ì¤Ê¤«¤Ã¤¿¤³¤È¤«¤é¡¤ÌÖÀÖ·ìµåΨ¤Î»»Äê¤Ï¹Ô¤ï¤Ê¤«¤Ã¤¿¡¥

5)ͺ¤Î·ì±ÕÀ¸²½³ØŪ¸¡ºº¡ÊTable 1¡Ë

¤¹¤Ù¤Æ¤ÎÅêÍ¿·²¤Ç¡¤Âоȷ²¤ËÈæ¤Ù̵µ¡¥ê¥ó¤ÎÁý²Ã¤¬Ç§¤á¤é¤ì¡¤¤µ¤é¤Ë150mg/kg°Ê¾å¤ÎÅêÍ¿·²¤Ç¥¯¥ì¥¢¥Á¥Ë¥ó¡¤Áí¥Ó¥ê¥ë¥Ó¥ó¡¤¥¢¥ë¥Ö¥ß¥ó¡¤A/G¤ª¤è¤Ó¥«¥ë¥·¥¦¥à¤ÎÁý²Ã¡¤GOT¤ª¤è¤Ó¦Ã-GTP³èÀ­¤Î¸º¾¯¤¬Ç§¤á¤é¤ì¤¿¡¥¤Þ¤¿¡¤750mg/kg·²¤Ç¤Ï¡¤ÁíÃÁÇò¤ÎÁý²Ã¡¤GPT³èÀ­¤ª¤è¤Ó±öÁǤθº¾¯¤¬¤½¤ì¤¾¤ìǧ¤á¤é¤ì¤¿¡¥¤½¤Î¾¡¤30¤ª¤è¤Ó150mg/kg·²¤Ç¥«¥ê¥¦¥à¤ÎÁý²Ã¤¬¡¤30mg/kg·²¤Ç¤Ï¦Ã-GTP³èÀ­¤ÎÁý²Ã¤¬Ç§¤á¤é¤ì¤¿¤¬¡¤ÍÑÎ̤˴ØÏ¢¤·¤¿ÊѲ½¤Ç¤Ï¤Ê¤«¤Ã¤¿¡¥

6)´ï´±½ÅÎÌ¡ÊTable 2, 3¡Ë

ͺ¤Ç¤Ï¡¤Âоȷ²¤ËÈæ¤Ù150¤ª¤è¤Ó750mg/kg·²¤Ç´Î¡¤ÎÁêÂнÅÎ̤¬Áý²Ã¤·¡¤¤µ¤é¤Ë750mg/kg·²¤Ç´Î¡¤Î¼Â½ÅÎÌ¡¤¿Õ¡¤Î¼Â½ÅÎ̤ª¤è¤ÓÁêÂнÅÎ̤¬Áý²Ã¤·¤¿¡¥¤½¤Î¾¡¤30mg/kg·²¤Ç¶»Á£½ÅÎ̤θº¾¯¤¬Ç§¤á¤é¤ì¤¿¤¬¡¤ÍÑÎ̤˴ØÏ¢¤·¤¿ÊѲ½¤Ç¤Ï¤Ê¤«¤Ã¤¿¡¥»ó¤Ç¤Ï¡¤Âоȷ²¤ËÈæ¤Ù750mg/kg·²¤Ç´Î¡¤Î¼Â½ÅÎ̤ª¤è¤ÓÁêÂнÅÎ̤¬¤È¤â¤ËÁý²Ã¤·¤¿¡¥

7)˶¸¡½ê¸«

ͺ¤Ç¤Ï¡¤´Î¡¤Î³ì¿§²½¤¬750mg/kg·²¤ÇÂоȷ²¤ËÈæ¤Ù¤Æȯ¸½ÉÑÅÙ¤ÎÍ­°Õ¤ÊÁý²Ã¤¬Ç§¤á¤é¤ì¤¿¡¥¤½¤Î¾¡¤´Î¡¤Îáíº¯¡¤¶»Á£¤ÎÀÖ¿§²½¡¤ÇÙ¤ÎÍ­¿§Èῶè°è¡Ê³ì¿§¡Ë¤ª¤è¤ÓÈé²¼Éô¤Î¼ðáñȯŪ¤Ëǧ¤á¤é¤ì¤¿¡¥»ó¤Ç¤Ï¡¤Ó®°é4Æü¤ÎÊìưʪ¤Ç¿Õ¡¤ÎÎп§²½¤¬150¤ª¤è¤Ó750mg/kg·²¤Ç¤½¤ì¤¾¤ì1Îã¤Ë´Ñ»¡¤µ¤ì¤¿¡¥¤½¤Î¾¡¤150¤Þ¤¿¤Ï750mg/kg·²¤Ç¶»Á£¤Î³ì¿§²½¡¤ÀÖ¿§Èῶè°è¤¢¤ë¤¤¤ÏÀÖ¿§²½¤ª¤è¤ÓÇÙ¤ÎÍ­¿§Èῶè°è¡Ê³ì¿§¡Ë¤¬Ã±È¯Åª¤Ëǧ¤á¤é¤ì¤¿¡¥

8)ÉÂÍýÁÈ¿¥³Ø½ê¸«¡ÊTable 4, 5¡Ë

ͺ¤Ç¤Ï¡¤¿Õ¡¤Ë¤ª¤¤¤ÆÇ¢ºÙ´É¾åÈé¤Î¹¥±ö´ðÀ­²½¡¤¾Ë»ÒÅ©ÊÑÀ­¤¬Âоȷ²¤ò´Þ¤à³Æ·²¤Ç´Ñ»¡¤µ¤ì¤¿¡¥¤Þ¤¿¡¤¿Õ¡¤Î¶á°ÌÇ¢ºÙ´É¾åÈé¤Î²õ»à¡¤±ó°ÌÇ¢ºÙ´É¤Î¹Ð³ÈÄ¥¤ª¤è¤ÓÀþ°Ý²½¤¬750mg/kg·²¤Ç´Ñ»¡¤µ¤ì¤¿¡¥´Î¡¤Ë¤ª¤¤¤Æ¤Ï¾®ÍÕÃæ¿´À­´ÎºÙ˦¼ðı¤ª¤è¤ÓÁý¿£Á㤬750mg/kg¤Ç´Ñ»¡¤µ¤ì¤¿¡¥¤Ê¤ª¡¤´ÎºÙ˦¤ÎÁý¿£Áã¤Îǧ¤á¤é¤ì¤¿Æ°Êª¤Ë¤ÏÈéÉæ¤ÎÀþ°ÝÆù¼ð¤Îʻ¸¤¬¤ß¤é¤ì¤¿¡¥¤½¤Î¾´Ñ»¡¤µ¤ì¤¿½ê¸«¤Ï¡¤Âоȷ²¤Èº¹¤¬¤Ê¤¤¤«¡¤¤¢¤ë¤¤¤ÏñȯÀ­¤ÎÉÂÊѤǤ¢¤Ã¤¿¡¥»ó¤Ç¤Ï¡¤ç£Â¡¤Î¿§ÁÇÄÀÃ太¤è¤Ó¤·ìж¿Ê¡¤¶»Á£¤Î½Ð·ì¤ª¤è¤Ó°à½Ì¡¤´Î¡¤Î»éËò½¡¦¾®Æù²êÁ㤪¤è¤Ó¿ñ³°Â¤·ìж¿Ê¡¤¿Õ¡¤ÎÀг¥ÄÀÃ塦ÃÁÇòÍͱßÃ줪¤è¤Ó±ó°ÌÇ¢ºÙ´É¤Î³ÈÄ¥¤ª¤è¤ÓÉû¿ÕÈé¼Á¤Î¶õ˦²½¤Ê¤É¤¬´Ñ»¡¤µ¤ì¤¿¤¬¡¤¤¤¤º¤ì¤âÂоȷ²¤Èº¹¤¬¤Ê¤¤¤«¡¤¤¢¤ë¤¤¤ÏñȯÀ­¤ÎÉÂÊѤǤ¢¤Ã¤¿¡¥

¸òÈø¤ÎÀ®Î©¤·¤Ê¤«¤Ã¤¿ÍºÆ°Êª¤Ç¤Ï¡¤750mg/kg·²¤Î1Îã¤ËÇ¥¿±¤òÀ®Î©¤µ¤»¤¿Æ°Êª¤ÈƱÍͤÎÊѲ½¤¬¿Õ¡¤ª¤è¤Ó´Î¡¤Ëǧ¤á¤é¤ì¤¿¡¥¤½¤Î¾¤Î½ê¸«¤Ï¡¤ç£Â¡¤Î¿§ÁÇÄÀÃ夢¤ë¤¤¤Ï¿Õ¡¤Î¹¥»ÀÀ­¾®ÂΤʤɼ«Á³È¯À¸À­ÉÂÊѤȤ·¤Æ¤·¤Ð¤·¤Ð¥é¥Ã¥È¤Ç´Ñ»¡¤µ¤ì¤ëÊѲ½¤Ç¤¢¤Ã¤¿¡¥¤Þ¤¿¡¤Ç¥¿±¤òÀ®Î©¤µ¤»¤Ê¤«¤Ã¤¿Íº¤ª¤è¤ÓÇ¥¿±ÉÔÀ®Î©¤Î»óưʪ¤Ç¤Ï¡¤È︳ʪ¼ÁÅêÍ¿¤Ë´ØÏ¢¤¹¤ëÆðÛŪ¤Ê¾ã³²¤ò¼¨º¶¤¹¤ë½ê¸«¤Ï¤ß¤é¤ì¤Ê¤«¤Ã¤¿¡¥

2.À¸¿£È¯À¸ÆÇÀ­

1)¸òÈø¤ª¤è¤Ó¼õÂÛǽ¡ÊTable 6¡Ë

¸òÈø¤Ï¡¤Âоȷ²¤ª¤è¤Ó30mg/kg·²¤ÇÁ´ÎãÀ®Î©¤·¤¿¡¥150¤ª¤è¤Ó750mg/kg·²¤Ç¤Ï¤½¤ì¤¾¤ì1ÁȤ¬¸òÈøÉÔÀ®Î©¤Ç¡¤¸òÈøΨ¤Ï¤½¤ì¤¾¤ì91.7¡ó¤Ç¤¢¤Ã¤¿¡¥¼õÂۤϡ¤Âоȷ²¡¤150¤ª¤è¤Ó750mg/kg·²¤Î¸òÈø¤¬À®Î©¤·¤¿»ó¤ÎÁ´Îã¤ÇÀ®Î©¤·¡¤30mg/kg·²¤Ç¤Ï12ÎãÃæ11Îã¡Ê91.7¡ó¡Ë¤ÇÀ®Î©¤·¤¿¡¥À­¼þ´ü´Ñ»¡¤Ç¤Ï¡¤¤¤¤º¤ì¤Î·²¤â¤Û¤Ü4¡Á5Æü¤ÎÀ­¼þ´ü¤ò¼¨¤·¤¿¤¬¡¤Âоȷ²¤ª¤è¤Ó150mg/kg·²¤Î³Æ1Îã¤Ëµ¶Ç¥¿±¤È¹Í¤¨¤é¤ì¤ëÀ­¼þ´ü¤ÎÄä»ß¡ÊϢ³¤·¤¿È¯¾ðµÙ»ß´üÁü¡Ë¤¬¸òÇÛľ¸å¤«¤éǧ¤á¤é¤ì¤¿¡¥¤³¤Î¤¦¤Á¡¤Âоȷ²¤Î1Îã¤ÏÀ­¼þ´ü¤ÎÄä»ß¤¬12Æü´Ö³¤¤¤¿¸å¸òÈø¤¬À®Î©¤·¤¿¤¬¡¤150mg/kg·²¤Î1Îã¤Ï¸òÇÛ´ü´Ö¤òÄ̤¸¤ÆÀ­¼þ´ü¤¬Ää»ß¤·¸òÈø¤ÎÀ®Î©¤Ë¤Ï»ê¤é¤Ê¤«¤Ã¤¿¡¥Ê¿¶ÑÀ­¼þ´ü¤ÏÂоȷ²¤Î4.6Æü¤ËÈæ¤Ù¤Æ750mg/kg·²¤Ç¤Ï4.1Æü¤Èû¤¯Åý·×³ØŪͭ°Õº¹¤¬Ç§¤á¤é¤ì¤¿¡¥

2)ʬÊÚ¤ª¤è¤ÓÓ®°é¡ÊTable 7¡Ë

ʬÊÚ¾õÂ֤ˤϰ۾ï¤Ï´Ñ»¡¤µ¤ì¤º¡¤³Æ·²¤ÎÇ¥¿±´ü´Ö¡¤²«Âοô¡¤Ãå¾²º¯¿ô¡¤½Ð»º»ù¿ô¡¤½Ð»ºÀ¸»ù¿ô¡¤À­È桤4ÆüÀ¸Â¸»ù¿ô¤ª¤è¤Ó»à»º»ù¿ô¤Ï¤Û¤ÜƱÍͤÊÃͤò¼¨¤·¡¤½Ð»ºÎ¨¡¤Ã徲Ψ¡¤Ê¬ÊÚΨ¡¤½ÐÀ¸Î¨¤ª¤è¤Ó4ÆüÀ¸Â¸Î¨¤Ë·²´Öº¹¤Ïǧ¤á¤é¤ì¤Ê¤«¤Ã¤¿¡¥

3)¿·À¸»ù¤Î·ÁÂÖ¡¤ÂνŤª¤è¤Ó˶¸¡½ê¸«

¿·À¸»ù¤Î³°É½¸¡ºº¤Ç¤Ï¡¤°Û¾ï¤Ï1Îã¤â´Ñ»¡¤µ¤ì¤Ê¤«¤Ã¤¿¡¥Ó®°é´ü´ÖÃæ¤ÎÂνŤϡ¤Âоȷ²¤ËÈæ¤Ù¤ÆÓ®°é4Æü¤ÎÂνŤ¬750mg/kg·²¤Îͺ¤ÇÁý²Ã·¹¸þ¤ò¼¨¤·¡¤»ó¤ÇÁý²Ã¤·¤¿¡¥Ó®°é4Æü¤Þ¤Ç¤Î»àË´»ù¤Î˶¸¡¤Ç¤Ï¡¤ÇÙ¤ÎÀÖ¿§²½¤¬Âоȷ²¤Î1Îã¤Ë´Ñ»¡¤µ¤ì¤¿¤Î¤ß¤Ç¤¢¤Ã¤¿¡¥Ó®°é4Æü¤Î˶¸¡¤Ç¤Ï¡¤¶»Á£¤ÎðôÉô»Äᤪ¤è¤Ó´Î¡¤Î´ñ·Á·ëÀá¡Ê²á·ÁÀ®¡Ë¤¬»¶¸«¤µ¤ì¤¿¡¥

¹Í»¡

1.È¿ÉüÅêÍ¿ÆÇÀ­

»àË´Îã¤Ï¡¤ÅêÍ¿´ü´Ö¤òÄ̤¸»óͺ¤¤¤º¤ì¤Î·²¤Ë¤âǧ¤á¤é¤ì¤Ê¤«¤Ã¤¿¡¥°ìÈ̾õÂ֤ˤâÈ︳ʪ¼ÁÅêÍ¿¤Î±Æ¶Á¤Ïǧ¤á¤é¤ì¤Ê¤«¤Ã¤¿¡¥

ÂνŤˤĤ¤¤Æ¤Ï¡¤Íº¤Î750mg/kg·²¤ÇÅêÍ¿´ü´Ö¤òÄ̤¸¤ÆÁý²ÃÍÞÀ©·¹¸þ¤¬Ç§¤á¤é¤ì¡¤·ÚÈù¤Ê¤¬¤éÈ︳ʪ¼ÁÅêÍ¿¤Î±Æ¶Á¤¬¹Í¤¨¤é¤ì¤¿¡¥°ìÊý¡¤»ó¤Ç¤ÏÈ︳ʪ¼ÁÅêÍ¿¤Î±Æ¶Á¤Ïǧ¤á¤é¤ì¤Ê¤«¤Ã¤¿¡¥ÀݱÂÎ̤ˤĤ¤¤Æ¤Ï¡¤Íº¤Ç¤ÏÈ︳ʪ¼ÁÅêÍ¿¤Î±Æ¶Á¤ò¼¨º¶¤¹¤ëÊѲ½¤Ïǧ¤á¤é¤ì¤Ê¤«¤Ã¤¿¡¥»ó¤Î750mg/kg·²¤ÎÇ¥¿±0¤«¤é14Æü¤Î´Ö¤ÇÁý²Ã¤¬Ç§¤á¤é¤ì¤¿¤¬¡¤È︳ʪ¼ÁÅêÍ¿¤È¤Î´ØÏ¢¤ÏÌÀ¤é¤«¤Ç¤Ê¤«¤Ã¤¿¡¥

·ì±Õ³ØŪ¸¡ºº¤Ç¤Ï¡¤È︳ʪ¼ÁÅêÍ¿¤Î±Æ¶Á¤Ïǧ¤á¤é¤ì¤Ê¤«¤Ã¤¿¡¥·ì±ÕÀ¸²½³ØŪ¸¡ºº¤Ç¤Ï¡¤150¤ª¤è¤Ó750mg/kg·²¤ÇÁíÃÁÇò¡¤¥¯¥ì¥¢¥Á¥Ë¥ó¤ª¤è¤ÓÁí¥Ó¥ê¥ë¥Ó¥ó¤ÎÁý²Ã¤¬Ç§¤á¤é¤ì¡¤¿Õ¡¤ª¤è¤Ó´Î¡¤Ø¤Î±Æ¶Á¤¬¼¨º¶¤µ¤ì¤¿¡¥¤½¤Î¾¡¤30mg/kg°Ê¾å¤ÎÅêÍ¿·²¤Ç̵µ¡¥ê¥ó¤ÎÁý²Ã¡¤150mg/kg°Ê¾å¤ÎÅêÍ¿·²¤Ç¥¢¥ë¥Ö¥ß¥ó¡¤A/G¤ª¤è¤Ó¥«¥ë¥·¥¦¥à¤ÎÁý²Ã¡¤GOT¤ª¤è¤Ó¦Ã-GTP³èÀ­¤Î¸º¾¯¡¤¤µ¤é¤Ë750mg/kg·²¤ÇGPT³èÀ­¤ª¤è¤Ó±öÁǤθº¾¯¤¬Ç§¤á¤é¤ì¤¿¤¬¡¤¤³¤ì¤é¤ÏÁê¸ß¤Ë´ØÏ¢¤·¤¿ÊѲ½¤Ç¤Ï¤Ê¤¯¡¤ÆÃÄê¡´ï¤Ë¤ª¤±¤ë¾ã³²¤ò¼¨º¶¤¹¤ë¤â¤Î¤È¤Ï¹Í¤¨¤é¤ì¤Ê¤«¤Ã¤¿¡¥

´ï´±½ÅÎ̤ϡ¤Íº¤Î150¤ª¤è¤Ó750mg/kg·²¤Ç´Î¡¤Î¼Â½ÅÎ̤ޤ¿¤ÏÁêÂнÅÎ̤¬¡¤¤µ¤é¤Ë750mg/kg·²¤Ç¿Õ¡¤Î¼Â½ÅÎ̤ª¤è¤ÓÁêÂнÅÎ̤¬Áý²Ã¤·¤¿¡¥»ó¤Ç¤Ï750mg/kg·²¤Ç´Î¡¤Î¼Â½ÅÎ̤ª¤è¤ÓÁêÂнÅÎ̤¬Áý²Ã¤·¤¿¡¥¤³¤ì¤é¤Î½ÅÎÌÊѲ½¤ÏÈ︳ʪ¼ÁÅêÍ¿¤Ëµ¯°ø¤·¤¿ÊѲ½¤È¹Í¤¨¤é¤ì¤¿¡¥

ÉÂÍý³Ø¸¡ºº¤Î·ë²Ì¡¤Æù´ã½ê¸«¤Ç¤Ïͺ¤Î750mg/kg·²¤Ç´Î¡¤Î³ì¿§²½¤¬¡¤»ó¤Î150¤ª¤è¤Ó750mg/kg·²¤Ç¿Õ¡¤ÎÎп§²½¤¬Ç§¤á¤é¤ì¤¿¤¬¡¤ÁÈ¿¥³Ø¸¡ºº¤Î·ë²Ì¤«¤é¤Ï³ì¿§²½¤¢¤ë¤¤¤ÏÎп§²½¤ò¼¨º¶¤¹¤ë½ê¸«¤Ïǧ¤á¤é¤ì¤º¡¤È︳ʪ¼ÁÅêÍ¿¤È¤Î´ØÏ¢¤ÏÉÔÌÀ¤Ç¤¢¤Ã¤¿¡¥ÁÈ¿¥³Ø½ê¸«¤Ç¤Ï¡¤Íº¤Ç¿Õ¡¤ÎÇ¢ºÙ´É¾åÈé¤Î¹¥±ö´ðÀ­²½¤ª¤è¤Ó¾Ë»ÒÅ©ÊÑÀ­¤¬Âоȷ²¤ò´Þ¤á³Æ·²¤Ë´Ñ»¡¤µ¤ì¤¿¡¥¤³¤ì¤é¤Ï¼«Á³È¯À¸À­¤ÎÊѲ½¤È¤·¤ÆÃΤé¤ì¤Æ¤¤¤ë¤¬£µ¡Ë,Ëܻ¤Ç¤Ï150mg/kg°Ê¾å¤ÎÅêÍ¿·²¤ÇÌÀ¤é¤«¤ËÍÑÎ̤ÎÁý²Ã¤Ëȼ¤Ã¤Æ¾ã³²¤ÎÄøÅÙ¤¬Áý¶¯¤·¡¤¤Þ¤¿750mg/kg·²¤Ç¶á°ÌÇ¢ºÙ´É¾åÈé¤Î²õ»à¡¤±ó°ÌÇ¢ºÙ´É¤Î³ÈÄ¥¤ª¤è¤ÓÀþ°Ý²½¤¬Ç§¤á¤é¤ì¤Æ¤¤¤ë¤³¤È¤ò¹Íθ¤¹¤ë¤È¡¤¿Õ¡¤Ø¤Î±Æ¶Á¤Î¤¢¤ë¤³¤È¤¬¼¨º¶¤µ¤ì¤¿¡¥¤·¤«¤·¡¤»ó¤Ç¤Ïͺ¤Çǧ¤á¤é¤ì¤¿¿Õ¡¤Î½ôÊѲ½¤Ïǧ¤á¤é¤ì¤Ê¤«¤Ã¤¿¡¥´Î¡¤Ç¤Ï¡¤Íº¤Î750mg/kg·²¤Ç·ÚÅ٤ξ®ÍÕÃæ¿´À­´ÎºÙ˦¼ðı¤¬Ç§¤á¤é¤ì¡¤´Î¡½ÅÎ̤ÎÁý²Ã¤È°ìÃפ·È︳ʪ¼ÁÅêÍ¿¤È¤Î´ØÏ¢¤¬¼¨º¶¤µ¤ì¤¿¡¥¤·¤«¤·¡¤»ó¤Ç¤Ï´Î¡½ÅÎ̤ÎÁý²Ã¤ò¼¨º¶¤¹¤ë½ê¸«¤Ïǧ¤á¤é¤ì¤Ê¤«¤Ã¤¿¡¥¤Þ¤¿¡¤´ÎºÙ˦Áý¿£Á㤪¤è¤ÓÈé²¼¤ÎÀþ°ÝÆù¼ð¤¬750mg/kg·²¤Ç1Îã¤ÎƱ°ì¸ÄÂΤ˴ѻ¡¤µ¤ì¤¿¤¬¡¤1Îã¤Î¤ß¤Ç¤¢¤êÈ︳ʪ¼ÁÅêÍ¿¤È¤Î´ØÏ¢¤Ë¤Ä¤¤¤Æ¤ÏȽÃǤǤ­¤Ê¤«¤Ã¤¿¡¥¤½¤Î¾¤Î½ê¸«¤Ï¡¤Ã±È¯À­¤¢¤ë¤¤¤Ï·²´Ö¤ËÌÀ¤«¤Êº¹¤Î¤Ê¤¤ÊѲ½¤Ç¤¢¤ê¡¤È︳ʪ¼ÁÅêÍ¿¤È¤Î´ØÏ¢¤Ï¤Ê¤¤¤â¤Î¤È¹Í¤¨¤é¤ì¤¿¡¥¾°¡¤È︳ʪ¼ÁÅêÍ¿¤Î±Æ¶Á¤Ï»ó¤ËÈæ¤Ù¤Æͺ¤Ç¶¯¤¯Ç§¤á¤é¤ì¤¿¤¬¡¤¤½¤Î¸¶°ø¤Ë¤Ä¤¤¤Æ¤ÏÌÀ¤é¤«¤Ç¤Ê¤«¤Ã¤¿¡¥

°Ê¾å¤Î¤³¤È¤«¤é¡¤2,2,4-¥È¥ê¥á¥Á¥ë-1,3-¥Ú¥ó¥¿¥ó¥¸¥ª¡¼¥ë¥¸¥¤¥½¥Ö¥Á¥ì¡¼¥È¤ÎÈ¿ÉüÅêÍ¿¤Ë¤è¤ëɸŪ´ï´±¤Ï¡¤´Î¡¤ª¤è¤Ó¿Õ¡¤È¹Í¤¨¤é¤ì¡¤Íº¤Ç¤Ï¡¤150mg/kg/day°Ê¾å¤ÎÅêÍ¿·²¤Ç¥¯¥ì¥¢¥Á¥Ë¥ó¤ª¤è¤ÓÁí¥Ó¥ê¥ë¥Ó¥ó¤ÎÁý²Ã¡¤´Î¡½ÅÎ̤ÎÁý²Ã¡¤¿Õ¡¤Ë¤ª¤±¤ëÇ¢ºÙ´É¾åÈé¤Î¹¥±ö´ðÀ­²½¤ÎÄøÅÙ¤ÎÁý²Ã¤ª¤è¤Ó¾Ë»ÒÅ©ÊÑÀ­¤¬¡¤¤µ¤é¤Ë¡¤750mg/kg/dayÅêÍ¿¤ÇÂνÅÁý²ÃÍÞÀ©¡¤ÁíÃÁÇò¡¤¥¯¥ì¥¢¥Á¥Ë¥ó¤ª¤è¤ÓÁí¥Ó¥ê¥ë¥Ó¥ó¤ÎÁý²Ã¡¤¿Õ¡¤Î½ÅÎÌÁý²Ã¡¤´Î¡¤Î³ì¿§²½¡¤¶á°ÌÇ¢ºÙ´É¾åÈé¤Î²õ»à¤Ê¤é¤Ó¤ËÀþ°Ý²½¡¤±ó°ÌÇ¢ºÙ´É¤Î³ÈÄ¥¡¤¾®ÍÕÃæ¿´À­´ÎºÙ˦¼ðı¤¬Ç§¤á¤é¤ì¤¿¡¥»ó¤Ç¤Ï¡¤750mg/kg/dayÅêÍ¿¤ÇÀݱÂÎ̤ÎÁý²Ã¤ª¤è¤Ó´Î¡½ÅÎ̤ÎÁý²Ã¤¬Ç§¤á¤é¤ì¤¿¡¥¤·¤¿¤¬¤Ã¤Æ¡¤Ìµ±Æ¶ÁÎ̤Ïͺ¤Ç¤Ï30mg/kg/day¡¤»ó¤Ç¤Ï150mg/kg/day¤ÈȽÃǤµ¤ì¤ë¡¥

2.À¸¿£È¯À¸ÆÇÀ­

¸òÈøǽ¤ª¤è¤Ó¼õÂÛǽ¤ËÈ︳ʪ¼ÁÅêÍ¿¤Î±Æ¶Á¤Ïǧ¤á¤é¤ì¤Ê¤«¤Ã¤¿¡¥À­¼þ´ü´Ñ»¡¤Ç¤Ï¡¤750mg/kg·²¤ÎÊ¿¶ÑÀ­¼þ´ü¤¬Âоȷ²¤Î4.6Æü¤ËÈæ¤Ù¤Æ4.1Æü¤Èû¤¯Åý·×³ØŪͭ°Õº¹¤¬Ç§¤á¤é¤ì¤¿¤¬¡¤Ê¿¶Ñ4.0Æü¤Îưʪ¤¬750mg/kg·²¤ËÊÒ´ó¤Ã¤¿¤³¤È¤Ë¤è¤ë¤â¤Î¤Ç¤¢¤ê¡¤Åö¥»¥ó¥¿¡¼¤ÎÇØ·ÊÃ͡ʲáµî3ǯ´Ö¡§Ê¿¶Ñ4.0¡Á4.4Æü¡Ë¤ÈÈæ³Ó¤·¤Æº¹¤Ï¤Ê¤«¤Ã¤¿¡¥Ê¬ÊÚ»þ´Ñ»¡¤Ç¤Ï¡¤Ç¥¿±Æ°Êª¤ÎÁ´Î㤬Àµ¾ï¤ËʬÊÚ¤·¡¤¿·À¸»ù¤Î³°É½¸¡ºº¤Ç¤âÈ︳ʪ¼ÁÅêÍ¿¤Î±Æ¶Á¤Ïǧ¤á¤é¤ì¤Ê¤«¤Ã¤¿¡¥¤Þ¤¿¡¤¿·À¸»ù¤ÎÀ¸Â¸Î¨¤Ë·²´Öº¹¤Ï¤Ê¤¯¡¤ÂνŤâÓ®°é4Æü¤Þ¤Ç½çÄ´¤ËÁý²Ã¤·¡¤»à»º»ù¡¤»àË´»ù¤ª¤è¤ÓÓ®°é4Æü¤Î˶¸¡¤Ç¤âÈ︳ʪ¼ÁÅêÍ¿¤Ë¤è¤ë±Æ¶Á¤Ïǧ¤á¤é¤ì¤Ê¤«¤Ã¤¿¡¥

°Ê¾å¤Î¤³¤È¤«¤é¡¤2,2,4-¥È¥ê¥á¥Á¥ë-1,3-¥Ú¥ó¥¿¥ó¥¸¥ª¡¼¥ë¥¸¥¤¥½¥Ö¥Á¥ì¡¼¥ÈÅêÍ¿¤Ë¤è¤ë»óͺ¤ÎÀ¸¿£¤ËµÚ¤Ü¤¹±Æ¶Á¤ª¤è¤Ó»ùưʪ¤ÎȯÀ¸¡¦È¯°é¤ËµÚ¤Ü¤¹±Æ¶Á¤Ï¡¤750mg/kg/dayÅêÍ¿¤Ë¤è¤Ã¤Æ¤âǧ¤á¤é¤ì¤º¡¤Ìµ±Æ¶ÁÎ̤ϤȤâ¤Ë750mg/kg/day¤ÈȽÃǤµ¤ì¤ë¡¥

ʸ¸¥

1¡ËGovt. Reports Announcements & Index, NTIS/PB 89-215776 (1982).
2¡ËC.G.Shayneand and S.W.Carrol,"Statistics and Experimental Design For Toxicologists," Telford Press, 1986.
3¡Ëº´µ×´Ö¾¼"Ìô¸úɾ²Á­µ¡Ý·×²è¤È²òÀÏ¡Ý," ÅìµþÂç³Ø½ÐÈDzñ, 1977.
4¡ËÀе¿Ê"À¸ÊªÅý·×³ØÆþÌç," ÇÝÉ÷´Û, 1975.
5¡ËH.Iwata, Y.Hirouchi,Y.Koike,S.Yamakawa,K.Kobayashi, T.Yamamoto, K.Kobayashi,H.Inoue and M.Enomoto; J.Toxicol.Pathol. 4;¡¡1-24,¡Ê1991¡Ë.

Ï¢ÍíÀè
»î¸³ÀÕǤ¼Ô¡§ÇëÅŧ°ì
»î¸³Ã´Åö¼Ô¡§ÅÄÃæμÂÀ¡¤ÅÏ¡¡½¤ÌÀ¡¤¾±»ÒÌÀÆÁ¡¤
×¢Æ⹯ɧ¡¤¾®ÎÓÏÂͺ
¡Êºâ¡Ë¿©ÉÊÇÀ°åÌôÉÊ°ÂÁ´À­É¾²Á¥»¥ó¥¿¡¼
¢©437-12ÀŲ¬¸©ÈØÅÄ·´Ê¡ÅÄÄ®±ö¿·ÅÄ»ú¹ÓÉÍ582-2
Tel 0538-58-1266Fax 81-538-58-1393

Correspondence
Authors:Koichi Hagita(Study director),
Ryota Tanaka, Nobuaki Watari, Akinori Shoji,
Yasuhiko Hirouchi, Kazuo Kobayashi
Biosafety Research Center, Foods, Drugs and Pesticides (An-Pyo Center)
582-2 Shioshinden Arahama, Fukude-cho, Iwata-gun, Shizuoka, 437-12, Japan
Tel +81-538-58-1266Fax +81-538-58-1393