n-Pentadecane was studied for oral toxicity in rats in a 28-day repeat dose toxicity test at doses of 0, 100, 300 and 1000 mg/kg/day and for mutagenicity with assays for reverse mutation in bacteria and chromosomal aberrations in cultured Chinese hamster (CHL) cells.

n-Pentadecane did not show any toxic effects in the repeat dose study. NOELs for repeat dose toxicity were 1000 mg/kg/day for both sexes.

n-Pentadecane was not mutagenic to S. typhimurium TA100, TA1535, TA98, TA1537 and E. coli WP2 uvrA with or without exogenous metabolic activation up to 5000 μg/plate. This chemical induced neither chromosomal aberrations nor polyploidy in CHL cells with or without exogenous metabolic activation up to 1.5 mg/ml.

n-Pentadecane[629-62-9]

1. Repeat Dose Toxicity 1)

Purity	:	99 %
Test species/strain	:	Rat/Crj:CD (SD)
Test method	:	Guidelines for 28-Day Repeat Dose Toxicity Test of Chemicals (Japan)
Route	:	Oral (gavage)
Doses	:	0 (vehicle), 100, 300, 1000 mg/kg/day
Number of animals	:	Male, 5; Female, 5
Vehicle	:	0.5 % CMC Na solution with 0.1% Tween 80
Administration period	:	Male and Female, 28 days
Terminal kill	:	Male and Female, days 29 or 43
GLP	:	Yes

　Test results:

No abnormal clinical signs or deaths were observed during the course of the study. There were no changes in body weight or food consumption. The test substance did not cause any differences from the controls in hematological analysis, blood biochemical analysis and urinalysis parameters.

There were no changes in organ weight. No abnormalities were seen that could be attributed to treatment with the test substance on macroscopic or microscopic pathological examinations.

NOEL: 1000 mg/kg/day

3. Genetic toxicity

3-1 Bacterial test 2)

Purity	:	> 99%
Test species/strain	:	S. typhimurium TA100, TA1535, TA98, TA1537 E. coli WP2 uvrA
Test method	:	Guidelines for Screening Mutagenicity Testing of Chemicals (Japan)
Procedure	:	Plate method
Solvent	:	Acetone
Positive control	:	-S9, AF-2 (TA100,WP2 uvrA,TA98), sodium azide (TA1525) and 9-aminoacridine (TA1537) +S9; 2-Aminoanthracene (all strains)
Doses	:	0, 312.5, 625, 1250, 2500, 5000 μg/plate
S-9	:	Rat liver, induced with phenobarbital and 5,6-benzoflavone
Plates/test	:	3
Number of replicate	:	2
GLP	:	Yes

　Test results:

Minimum concentration of test substance at which toxicity to bacteria was observed:
No toxicity was observed up to a concentration of 5000 μg/plate with or without metabolic activation.

Genotoxic effects:
S. typhimurium TA100, TA1535, TA98, TA1537

	+	?	-
with metabolic activation:	[ ]	[ ]	[*]
without metabolic activation:	[ ]	[ ]	[*]

E. coli WP2 uvrA

	+	?	-
with metabolic activation:	[ ]	[ ]	[*]
without metabolic activation:	[ ]	[ ]	[*]

3-2 Non-bacterial in vitro test (Chromosomal aberration) 3)

Purity	:	99 %
Type of cell used	:	Chinese hamster CHL cells
Test method	:	Guidelines for Screening Mutagenicity Testing of Chemicals (Japan)
Solvent	:	Acetone
Positive controls	:	-S9, Mitomycin C +S9, Cyclophosphamide
Doses	:	-S9, 0, 0.37, 0.75, 1.5 mg/ml +S9, 0, 0.37, 0.75, 1.5 mg/ml
S-9	:	Rat liver, induced with phenobarbital and 5,6-benzoflavone
Plates/test	:	2
GLP	:	Yes

　Test results:

n-Pentadecane induced neither chromosomal aberrations nor polyploidy up to a concentration of 1.5 mg/ml with or without exogenous activation.

Lowest concentration producing cytogenetic effects in vitro:
with metabolic activation: > 1.5 mg/ml
without metabolic activation: > 1.5 mg/ml

Genotoxic effects:

	+	?	-
with metabolic activation:	[ ]	[ ]	[*]
without metabolic activation:	[ ]	[ ]	[*]

1)	The test was performed by the Biosafety Research Center, Foods, Drugs and Pesticide (An-pyo Center), 582-2 Shioshinden Arahama, Fukude-cho, Iwata-gun, Shizuoka, 437-12, Japan. Tel 81-538-58-1266 Fax 81-538-58-1393
2)	The tests were performed by the Hatano Research Institute, Food and Drug Safety Center, 729-5 Ochiai, Hadano-shi, Kanagawa, 257, Japan. Tel 81-463-82-4751 Fax 81-463-82-9627