2-Hydroxy-4-(octyloxy)benzophenone

2-ヒドロキシ-4-(オクチルオキシ)ベンゾフェノン

[CAS No. 1843-05-6]

Molecular formula: C21H26O3 Molecular weight: 326.44

ABSTRACT

2-Hydroxy-4-(octyloxy)benzophenone was studied for oral toxicity in rats in a 28-day repeat dose toxicity test at doses of 0, 20, 140, and 1000 mg/kg/day. In the repeat dose study, no test substance-related changes were noted in terms of clinical observation, body weights, food consumption, and the findings obtained from hematology tests, blood chemical examination, urinalysis and pathological examination. The NOEL for the repeat dose toxicity is considered to be 1000 mg/kg/day for both sexes.

2-Hydroxy-4-(octyloxy)benzophenone was not mutagenic to Salmonella typhimurium, TA100, TA1535, TA98, TA1537 and Escherichia coli WP2 uvrA. Neither structural nor numerical chromosomal aberrations were induced in CHL/IU cells up to the limit concentration of 5 mg/ml, in the absence or presence of an exogeneous metabolic activation system.

SUMMARIZED DATA FROM THE STUDIES

2. Repeat Dose Toxicity 1)

Purity:> 99%
Test species/strain:Rat/Crj:CD (SD)
Test method:Guidelines for 28-Day Repeat Dose Toxicity Testing for Chemicals (Japan)
　Route:Oral (gavage)
　Doses:0 (vehicle), 20, 140, 1000 mg/kg/day
　Number of animals/group:Males, 6 ; females, 6
　Vehicle:0.5% sodium carboxymethyl cellulose solution containing 0.1% Tween 80
　Administration period:Males and females, 28 days
　Terminal kill:Days 29 or 43
GLP:Yes

　 Test results:

No test substance-related changes were noted on clinical observation or in body weights, food consumption, and findings obtained from hematology tests, blood chemical examination, urinalysis and pathological examination.
The NOEL for the repeat dose toxicity is considered to be 1000 mg/kg/day for both sexes.

2. Genetic Toxicity

2-1. Bacterial test 2)

Purity:≧ 99%
Test species/strains:Salmonella typhimurium TA100, TA1535, TA98, TA1537,
Escherichia coli WP2 uvrA
Test methods:OECD guideline (No. 471, 472) and Guidelines for Screening Mutagenicity Testing of Chemicals (Japan)
　Procedures:Pre-incubation method
　Solvent:DMSO
　Positive controls:-S9 mix, AF-2 (TA100, TA98), sodium azide (TA1535), ENNG (WP2 uvrA) and 9-aminoacridine (TA1537) +S9 mix, 2-aminoanthracene (all strains)
　Doses:313, 625, 1250, 2500, 5000μg/plate
　S9:Rat liver, induced with phenobarbital and 5,6-benzoflavone
　Plates/test:3
　Number of replicates:2
GLP:Yes

　 Test results:

This chemical did not induce gene mutations in the S. typhimurium and E. coli strains. No toxicity was observed up to a concentration of 5000 μg/plate with or without metabolic activation.

Genetic effects:
Salmonella typhimurium TA100, TA1535, TA98, TA1537
+?-
without metabolic activation[ ][ ][*]
with metabolic activation[ ][ ][*]

E. coli WP2 uvrA
+?-
without metabolic activation[ ][ ][*]
with metabolic activation[ ][ ][*]

2-2. Non-bacterial in vitro test (chromosomal aberration test) １）

Purity:≧99%
Type of cell used:Chinese hamster CHL/IU cells
Test method:OECD guideline (No. 473) and Guidelines for Screening Mutagenicity Testing of Chemicals (Japan)
　Solvent:Acetone
　Positive controls:-S9 mix, Mitomycin C
+S9 mix, Benzo[a]pyrene
　Doses:-S9 mix (24 h treatment): 0, 1250, 2500, 5000 μg/ml
-S9 mix (48 h treatment): 0, 1250, 2500, 5000 μg/ml
-S9 mix (6 h pulse treatment): 0, 1250, 2500, 5000 μg/ml
+S9 mix (6 h pulse treatment): 0, 1250, 2500, 5000 μg/ml
　S-9:Rat liver, induced with phenobarbital and 5,6-benzoflavone
　Plates/test:2
GLP:Yes

　 Test results:

No structural or numerical chromosomal aberrations were induced under the experimental conditions used.

Genotoxic effects:
clastogenicitypolyploidy
+?-+?-
without metabolic activation:[ ][ ][*][ ][ ][*]
with metabolic activation:[ ][ ][*][ ][ ][*]

1)The tests were performed by the Mitsubishi Chemical Safety Institute Ltd., 14 Sunayama, Hasaki-machi, Kashima-gun, Ibaraki, 314-02, Japan. Tel +81-479-46-2871 Fax +81-479-46-2874