2-Vinylpyridine

2-ビニルピリジン


[CAS No. 100-69-6]

2-Ethenylpyridine

2-エテニルピリジン

Molecular formula: C7H7N Molecular weight: 105.15

ABSTRACT

2-Vinylpyridine was studied for oral toxicity in rats in 28-day repeat dose toxicity test at doses of 0, 12.5, 50 and 200 mg/kg/day. Salivation was observed in both sexes receiving 50 and 200 mg/kg. Body weight gain was suppressed and food consumption decreased in males receiving 200 mg/kg. Urinalysis revealed decreases in specific gravity in females receiving 50 and 200 mg/kg and volume increase in females receiving 200 mg/kg. Relative testes weights were increased in the males receiving 200 mg/kg. Absolute and relative spleen weights were decreased and relative liver weights increased in females receiving 200 mg/kg. Squamous hyperplasia in the forestomach was observed in the both sexes receiving 50 and 200 mg/kg along with thickening of the mucosa at the higher dose. The NOEL for repeat dose toxicity is considered to be 12.5 mg/kg/day for both sexes.

2-Vinylpyridine was mutagenic to E. coli WP2 uvrA with an exogeneous metabolic activation system.

2-Vinylpyridine induced structural chromosomal aberrations in CHL cells, in the absence or presence of an exogenous metabolic activation system.

SUMMARIZED DATA FROM THE STUDIES

1. Repeat Dose Toxicity1)

Purity:98.3%
Test species/strain:Rats/Crj: CD (SD)
Test method:Guidelines for 28-day Repeat Dose Toxicity Testing of Chemicals (Japan)
 Route:Oral
 Doses:0 (vehicle), 12.5, 50, 200 mg/kg/day
 Number of animals:Males, 5; females,5/group
 Vehicle:Corn oil
 Administration period:Males and females, 28 days
 Terminal killing:Males and females, days 29 or 43
GLP:Yes
 Test results:

Salivation was observed in both sexes receiving 50 and 200 mg/kg. Body weight gain was suppressed and food consumption decreased in males receiving 200 mg/kg. Urinalysis revealed decreases in specific gravity in females receiving 50 and 200 mg/kg and volume increase in females receiving 200 mg/kg. Relative testes weights were increased in males receiving 200 mg/kg. Absolute and relative spleen weights were decreased and relative liver weights increased in females receiving 200 mg/kg. Squamous hyperplasia and submucosal edema in the forestomach were observed in both sexes receiving 50 and 200 mg/kg, along with thickening of the mucosa at the higher dose. Moreover, erosion and cellular infiltration in the forestomach were observed in males receiving 200 mg/kg. Submucosal edema and/or erosion in the glandular stomach were also observed in females receiving 50 or 200 mg/kg.

Squamous hyperplasia in the forestomach was still observed in both sexes receiving 200 mg/kg at the end of recovery period, but its incidence and extent were decreased in comparison to those of the same groups at the end of administration period.

The NOEL for repeat dose toxicity is considered to be 12.5 mg/kg/day for both sexes.

2. Genetic Toxicity

2-1. Bacterial test1)

Purity:98.3%
Test species/strains:S. typhimurium TA100, TA1535, TA98, TA1537, E. coli WP2 uvrA
Test method:Guidelines for Screening Mutagenicity Testing of Chemicals (Japan)
 Procedures:Pre-incubation method
 Solvent:DMSO
 Positive controls:-S9 mix; AF-2 (TA100, TA98 and WP2 uvrA), Sodium azide (TA1535) and 9-Aminoacridine (TA1537)
+S9 mix; 2-Aminoanthracene (all strains)
 Doses:-S9 mix; 39.1, 78.1, 156, 313, 625, 1250 and 2500 μg/plate (TA100 and TA1535)
156, 313, 625, 1250, 2500 and 5000 μg/plate (TA98,TA1537 and WP2 uvrA)
+S9 mix; 156, 313, 625, 1250, 2500 and 5000 μg/plate
+S9 mix (WP2 uvrA; confirmative examination); 2500, 3000, 3500, 4000, 4500 and 5000 μg/plate
 S9:Rat liver, induced with phenobarbital and 5,6-benzoflavone
 Plates/test:3
 Number of replicates:2
GLP:Yes
 Test results:
Clear positive mutagenic responses were obtained in E. coli WP2 uvrA with metabolic activation.

Genetic effects:
S. typhimurium TA100, TA1535, TA98 and TA1537
+?-
Without metabolic activation:[ ][ ][*]
With metabolic activation:[ ][ ][*]

E. coli WP2 uvrA
+?-
Without metabolic activation:[ ][ ][*]
Without metabolic activation:[*][ ][ ]

2-2. Non-bacterial in vitro test (chromosomal aberration test)1)

Purity:98.3%
Type of cell used:Chinese hamster lung (CHL) cells
Test method:Guidelines for Screening Mutagenicity Testing of Chemicals (Japan)
 Solvent:DMSO
 Positive controls:-S9 mix, Mitomycin C
+S9 mix, Cyclophosphamide
 Doses:-S9 mix (continuous treatment):-S9 mix (24h continuous exposure): 0, 3.75, 7.50, 15.0 μg/ml
-S9 mix (48h continuous exposure): 0, 1.88, 3.75, 7.50 μg/ml
-S9 mix (short-term exposure): 0, 15.0, 30.3, 60.0 μg/ml
+S9 mix (short-term exposure): 0, 37.5, 75.0, 150 μg/ml
 S9:Rat liver, induced with phenobarbital and 5,6-benzoflavone
 Plates/test:2
GLP:Yes

 Test results:
This chemical induced structural chromosomal aberrations in the absence or presence of an exogenous metabolic activation system.

Lowest concentration producing cytogenetic effects in vitro:

Without metabolic activation (24h treatment): 0.00557 mg/ml (clastogenicity)

Genotoxic effects:
clastogenicitypolyploidy
+?- +?-
Without metabolic activation:[*][ ][ ][ ][ ][*]
With metabolic activation:[*][ ][ ][ ][ ][*]

1)The tests were performed by the Biosafety Research Center, Foods, Drugs and Pesticides (An-pyo Center), Japan, 582-2 Shioshinden Arahama, Fukude-cho, Iwata-gun, Shizuoka, 437-12, Japan. Tel +81-538-58-1266 Fax +81-538-58-1393